DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Arum | ko |
dc.contributor.author | Park, Eun Byeol | ko |
dc.contributor.author | Lee, Janghyun | ko |
dc.contributor.author | Choi, Byong-Seok | ko |
dc.contributor.author | Kang, Suk-Jo | ko |
dc.date.accessioned | 2017-05-08T08:48:39Z | - |
dc.date.available | 2017-05-08T08:48:39Z | - |
dc.date.created | 2017-03-13 | - |
dc.date.issued | 2017-03 | - |
dc.identifier.citation | FEBS LETTERS, v.591, no.6, pp.954 - 961 | - |
dc.identifier.issn | 1873-3468 | - |
dc.identifier.uri | http://hdl.handle.net/10203/223513 | - |
dc.description.abstract | Cyclic GMP-AMP synthase (cGAS) is a DNA-sensing enzyme in the innate immune system. Recent studies using core-cGAS lacking the N terminus investigated the mechanism for binding of double-stranded (ds) DNA and synthesis of 2 ',3 '-cyclic GMP-AMP (cGAMP), a secondary messenger that ultimately induces type I interferons. However, the function of the N terminus of cGAS remains largely unknown. Here, we found that the N terminus enhanced the activity of core-cGAS in vivo. Importantly, the catalytic activity of core-cGAS decreased as the length of double-stranded DNA (dsDNA) increased, but the diminished activity was restored by addition of the N terminus. Furthermore, the N terminus de-oligomerized the 2 : 2 complex of core-cGAS and dsDNA into a 1 : 1 complex, suggesting that the N terminus enhanced the activity of core-cGAS by facilitating formation of a monomeric complex of cGAS and DNA. | - |
dc.language | English | - |
dc.publisher | WILEY | - |
dc.subject | CYCLIC GMP-AMP | - |
dc.subject | INNATE IMMUNE SENSOR | - |
dc.subject | SYNTHASE | - |
dc.subject | 2ND-MESSENGER | - |
dc.subject | DINUCLEOTIDE | - |
dc.title | The N terminus of cGAS de-oligomerizes the cGAS: DNA complex and lifts the DNA size restriction of core-cGAS activity | - |
dc.type | Article | - |
dc.identifier.wosid | 000398003200012 | - |
dc.identifier.scopusid | 2-s2.0-85014663598 | - |
dc.type.rims | ART | - |
dc.citation.volume | 591 | - |
dc.citation.issue | 6 | - |
dc.citation.beginningpage | 954 | - |
dc.citation.endingpage | 961 | - |
dc.citation.publicationname | FEBS LETTERS | - |
dc.identifier.doi | 10.1002/1873-3468.12598 | - |
dc.contributor.localauthor | Choi, Byong-Seok | - |
dc.contributor.localauthor | Kang, Suk-Jo | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
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