Expanding the genetic code of Mus musculus

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Here we report the expansion of the genetic code of Mus musculus with various unnatural amino acids including Ne-acetyl-lysine. Stable integration of transgenes encoding an engineered Ne-acetyl-lysyl-tRNA synthetase (AcKRS)/tRNAPyl pair into the mouse genome enables site-specific incorporation of unnatural amino acids into a target protein in response to the amber codon. We demonstrate temporal and spatial control of protein acetylation in various organs of the transgenic mouse using a recombinant green fluorescent protein (GFPuv) as a model protein. This strategy will provide a powerful tool for systematic in vivo study of cellular proteins in the most commonly used mammalian model organism for human physiology and disease.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2017-02
Language
English
Article Type
Article
Keywords

MESSENGER-RNA DECAY; TRANSCRIPTION; CHEMISTRY; PROTEINS; BIOLOGY; CANCER; MTDNA

Citation

NATURE COMMUNICATIONS, v.8

ISSN
2041-1723
DOI
10.1038/ncomms14568
URI
http://hdl.handle.net/10203/222730
Appears in Collection
CH-Journal Papers(저널논문)
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