Effects of a MTND1 mutation on the hepatic metabolism in human induced pluripotent stem cell-derived hepatocytes = MTND1 돌연변이가 유도만능줄기세포 유래 간세포의 대사에 미치는 영향

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dc.contributor.advisorHan, Yong-Mahn-
dc.contributor.advisor한용만-
dc.contributor.authorIm, Ilkyun-
dc.contributor.author임일균-
dc.date.accessioned2017-03-29T02:45:08Z-
dc.date.available2017-03-29T02:45:08Z-
dc.date.issued2016-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=648154&flag=dissertationen_US
dc.identifier.urihttp://hdl.handle.net/10203/222130-
dc.description학위논문(박사) - 한국과학기술원 : 생명과학과, 2016.2 ,[viii, 109 p. :]-
dc.description.abstractDysfunction of oxidative phosphorylation (OXPHOS) that takes place in mitochondria causes various metabolic disorders in human. However the metabolism of liver in complex I defect was poorly investigated so far. Here I show that hepatic metabolism was changed by energy depletion which causes aggravation of lactic acidosis through AMP-activated protein kinase (AMPK)-dependent pathway in hiPSC-derived hepatocytes from patient having homoplasmic m.3398T>C on mitochondrial-encoded NADH dehydrogenase 1 (MTND1). This in vitro cellular model revealed aberrant mitochondrial characteristics including their morphologies and biogenesis. In addition, glycogen storage level was significantly decreased in patient-derived hepatocytes. Patient iPSC-derived hepatocytes showed decreased Complex I activity, cellular ATP level and NAD+/NADH ratio which mean dysfunction of OXPHOS. Intriguingly, genes related to the conversion of lactate into other metabolite such as glucose and alanine were significantly decreased in patient iPSC-derived hepatocytes. This phenomenon would accelerate lactic acidosis in the patient. Metabolic changes in glycogen storage and lactate turnover were driven by activation of AMPK which is positively regulated by ATP depletion. I rescued phenotypes in glycogen and lactate metabolism by treating an AMPK inhibitor and small interfering RNA (siRNA). These results imply that energy depletion in hepatocytes may result in metabolic shift via AMPK.-
dc.languageeng-
dc.publisher한국과학기술원-
dc.subjectMTND1 defect-
dc.subjectinduced pluripotent stem cells-
dc.subjecthepatocytes-
dc.subjectlactic acidosis-
dc.subjectAMPK-
dc.subjectcellular modeling-
dc.subjectMTND1 결손-
dc.subject유도만능줄기세포-
dc.subject간세포-
dc.subject젖산 혈증-
dc.subject세포 모델링-
dc.titleEffects of a MTND1 mutation on the hepatic metabolism in human induced pluripotent stem cell-derived hepatocytes = MTND1 돌연변이가 유도만능줄기세포 유래 간세포의 대사에 미치는 영향-
dc.typeThesis(Ph.D)-
dc.identifier.CNRN325007-
dc.description.department한국과학기술원 :생명과학과,-
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