Roles of 14-3-3 and Tctp for organ growth in Drosophila14-3-3 및 Tctp 단백질의 초파리 기관성장에서의 역할

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14-3-3 family proteins regulate multiple signaling pathways. Understanding biological functions of 14-3-3 proteins has been limited by the functional redundancy of conserved isotypes. Here, we provide evidence that 14-3-3 proteins regulate two interacting components of Tor signaling in Drosophila, Translationally controlled tumor protein (Tctp) and Rheb GTPase. Single knockdown of $14-3-3 \varepsilon$ or $14-3-3 \zeta$ isoform does not show obvious defects in organ development but causes synergistic genetic interaction with Tctp and Rheb to impair tissue growth. 14-3-3 proteins physically interact with Tctp and Rheb. Knockdown of both 14-3-3 isoforms abolishes the binding between Tctp and Rheb, disrupting organ development. Depletion of 14-3-3s also reduces the level of phosphorylated S6 kinase, phosphorylated Thor/4E-BP and cyclin E (CycE). Growth defects from knockdown of 14-3-3 and Tctp are suppressed by CycE overexpression. This study suggests a novel mechanism of Tor regulation mediated by 14-3-3-dependent regulation of Tctp and Rheb.
Advisors
Choi, Kwang Wookresearcher최광욱researcher
Description
한국과학기술원 :생명과학과,
Publisher
한국과학기술원
Issue Date
2016
Identifier
325007
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 생명과학과, 2016.8 ,[vi, 84 p. :]

Keywords

14-3-3; Tctp; Rheb; cell cycle; signaling

URI
http://hdl.handle.net/10203/222125
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=663106&flag=dissertation
Appears in Collection
BS-Theses_Ph.D.(박사논문)
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