DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Song, Ji-Joon | - |
dc.contributor.advisor | 송지준 | - |
dc.contributor.author | Park, Jihye | - |
dc.contributor.author | 박지혜 | - |
dc.date.accessioned | 2017-03-29T02:44:49Z | - |
dc.date.available | 2017-03-29T02:44:49Z | - |
dc.date.issued | 2014 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=657430&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/222111 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2014.2 ,[v, 96 p. :] | - |
dc.description.abstract | Host cell factor 1 (HCF-1) is a highly conserved transcriptional regulator that participates in formation of various transcription regulatory complexes. HCF-1 undergoes an unusual proteolytic maturation process to generate $HCF-1_N$ and $HCF-1_C$ subunits that remain noncovalently associated via self-association sequence elements. Here, I present the crystal structure of the self-association sequence 1 (SAS1) and the C-terminal nuclear localization signal (NLS) showing that the SAS1 elements from each of the $HCF-1_N$ and $HCF-1_C$ promotes the association by forming an interdigitated fibronectin type 3 (Fn3) tandem repeat structure. I also show that the C-terminal NLS is required for effective formation of a transcriptional regulatory complex: the herpes simplex virus VP16-induced complex formation. Interestingly, the basic residues of NLS play critical role in both VIC formation and nuclear localization. Thus, this study reveals that the role of self-association via an interdigitated Fn3 structure is to hold the $HCF-1_N$ and $HCF-1_C$ subunits together to facilitate a transcriptional regulatory complex formation by recruiting the NLS. In addition, these results present a novel function of NLS to promote the VIC formation suggesting that the NLS of HCF-1 has dual function in localizing HCF-1 to the nucleus and promoting VIC formation. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | crystallography | - |
dc.subject | HCF-1 | - |
dc.subject | self-association | - |
dc.subject | VP16-induced complex | - |
dc.subject | nuclear localization signal | - |
dc.subject | 구조학 | - |
dc.subject | 자가 결합 | - |
dc.title | Structural and biochemical studies on the mechanism and the role of HCF-1 self-association and nuclear localization signal in VP16-induced complex formation | - |
dc.title.alternative | HCF-1 단백질의 자가 조립을 통한 유전자 발현 복합체의 형성 촉진에 대한 구조 생화학적 연구 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :생명과학과, | - |
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