DC Field | Value | Language |
---|---|---|
dc.contributor.advisor | Koh, Gou Young | - |
dc.contributor.advisor | 고규영 | - |
dc.contributor.advisor | Kim, Ho Min | - |
dc.contributor.advisor | 김호민 | - |
dc.contributor.author | Oh, Nuri | - |
dc.contributor.author | 오누리 | - |
dc.date.accessioned | 2017-03-29T02:44:24Z | - |
dc.date.available | 2017-03-29T02:44:24Z | - |
dc.date.issued | 2016 | - |
dc.identifier.uri | http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=648149&flag=dissertation | en_US |
dc.identifier.uri | http://hdl.handle.net/10203/222085 | - |
dc.description | 학위논문(박사) - 한국과학기술원 : 생명과학과, 2016.2 ,[v, 63 p. :] | - |
dc.description.abstract | Angiopoietin-1 (Ang1), a potential growth factor for therapeutic angiogenesis and vascular stabilization, is known to specifically cluster and activate Tie2 in high oligomeric forms, which is a unique and essential process in this ligand-receptor interaction. However, highly oligomeric native Ang1 and Ang1 variants are difficult to produce, purify, and store in a stable and active form. To overcome these limitations, we developed a simple and active dimeric CMP-Ang1 by replacing the N-terminal of native Ang1 with the coiled-coil domain of cartilage matrix protein (CMP) bearing mutations in its cysteine residues. This dimeric CMP-Ang1 effectively increased the migration, survival, and tube formation of endothelial cells via Tie2 activation. Furthermore, dimeric CMP-Ang1 induced angiogenesis and sup-pressed vascular leakage in vivo. Despite its dimeric structure, the potencies of such Tie2-activation-induced effects were comparable to those of a previously engineered protein, COMP-Ang1. We also revealed that these effects of dimeric CMP-Ang1 were affected by specified N-glycosylation in its fibrinogen-like domain. Taken together, our results indicate that dimeric CMP-Ang1 is capable of activating Tie2 and stimulating angiogenesis in N-glycan dependent manner. | - |
dc.language | eng | - |
dc.publisher | 한국과학기술원 | - |
dc.subject | Angiopoietin-1 | - |
dc.subject | Tie2 | - |
dc.subject | CMP-Ang1 | - |
dc.subject | dimeric-Ang1 | - |
dc.subject | Tie2 수용체 | - |
dc.subject | 이중체 Ang1 | - |
dc.title | Development of dimeric CMP-Ang1 activating angiogenesis and vessel stabilization | - |
dc.title.alternative | 혈관 신생을 유도하는 단백질 신약 이중체 CMP-Ang1의 개발에 관한 연구 | - |
dc.type | Thesis(Ph.D) | - |
dc.identifier.CNRN | 325007 | - |
dc.description.department | 한국과학기술원 :생명과학과, | - |
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