Overproduction of free fatty acids from rhodococcus opacus strain PD630 for biodiesel production = 바이오디젤 생산을 위한 로도코코스 균에서의 유리지방산의 과생산

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 284
  • Download : 0
Free fatty acids are merely carboxylic acids with long hydrocarbon chains. They are produced when fats are broken and considered as good fats. Free fatty acids can be used as precursors of fatty acid methyl esters (FAMEs) which are used as diesel. Currently, alternative fuels of which feedstock is originated in the nature or which was made by biological methods have been greatly interested as they do not increase the net carbon dioxide, one of the greenhouse gases that enhance radioactive force and contribute to global warming. Rhodococcus opacus PD630 is able to accumulate lipids up to 76 or 87% of the cellular dry mass as triacylglycerides (TAG), diacylglycerides (DAG) and wax esters etc. respectively. To produce free fatty acids using these lipids, the genes encoding TAG lipase, monoacylglyceride (MAG) lipase from other organisms were overexpressed in R. opacus PD630 using the R. opacus PD630-Escherichia coli shuttle vectors. For accumulating lipids in R. opacus PD630, mineral salt media (MSM) cultivation was optimized and MSM cultivation without nitrogen source was used. Produced free fatty acids from these cultures were chemically converted into FAMEs by methylation. As a result, the mutant R. opacus PD630 strain was able to produce free fatty acids with 50% of free ratty acids yield from glucose in contrast with R. opacus PD630 wild strain having ability to produce with 33.5% of free fatty acids yield from glucose. From these fatty acids, FAME ?? diesel was produced by chemical method and the concentration was 5.393g/l using free fatty acids which were produced from fed-batch fermentation.
Advisors
Lee, Sang Yupresearcher이상엽researcher
Description
한국과학기술원 :생명화학공학과,
Publisher
한국과학기술원
Issue Date
2010
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2010.8 ,[iii, 66 p. :]

Keywords

Biodiesel; free fatty acid; Rhodococcus opacus PD630; microbial diesel; fatty acid methyl ester; 바이오디젤; 유리지방산; 로도코코스; 미생물 디젤; 지방산 메틸 에스터

URI
http://hdl.handle.net/10203/221509
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=656993&flag=dissertation
Appears in Collection
CBE-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0