Enzymatic biotransformation of ginsenosides $Rb_1$, $Rb_2$, Rc and Rd into $F_2$ by novel recombinant ginsenoside hydrolyzing glycosidase from Novosphingobium aromaticivoransNovosphingobium aromaticivorans로부터의 새로운 재조합 가수분해 효소를 이용한 진세노사이드 $Rb_1$, $Rb_2$, Rc, Rd의 $F_2$ 로의 생물전환

Cited 0 time in webofscience Cited 0 time in scopus
  • Hit : 842
  • Download : 1
DC FieldValueLanguage
dc.contributor.advisorKim, Sun Chang-
dc.contributor.advisor김선창-
dc.contributor.authorJo, Young Jun-
dc.contributor.author조영준-
dc.date.accessioned2017-03-29T02:33:40Z-
dc.date.available2017-03-29T02:33:40Z-
dc.date.issued2016-
dc.identifier.urihttp://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=649467&flag=dissertationen_US
dc.identifier.urihttp://hdl.handle.net/10203/221471-
dc.description학위논문(석사) - 한국과학기술원 : 생명과학과, 2016.2 ,[v, 34 p. :]-
dc.description.abstractThis study focuses on the cloning, expression, and characterization of a recombinant ginsenoside hy-drolyzing glycosidase from Novosphingobium aromaticivorans KCTC $2888^{T}$ in order to efficiently biotransform protopanaxadiol type major ginsenosides ($Rb_1$, $Rb_2$, Rc, Rd) into ginsenoside $F_2$. The gene (BglNar) consists of 1,320 bp (439 amino acid residues) and revealed significant homology to that of glycoside hydrolase family 1. The gene was cloned and the recombinant enzyme overexpressed in Escherichia coli BL21(DE3) using a GST-fused pGEX 4T-1 vector system was characterized at conditions of pH 6.0 and $37^\circ C$. BglNar could convert gin-senosides $Rb_1$, $Rb_2, Rc$ into $F_2$ via Rd or gypenoside XVII, Rd, $C-Mc_1$, respectively, through selective hydrolysis of the glucose or arabinosides moieties. $K_m$ value of p-nitrophenyl- $\beta$ -D-glucopyranoside was 6.73 $\pm$ 0.27 and the $V_{max}$ value was 21.2 $\pm$ 0.36 $\mu mol min^{-1} mg^{-1}$ of protein, respectively. A crude protopanaxadiol-type ginsenoside mixture (PPDGM) was treated with BglNar followed by a column purification packed with HP20 resin, to pro-duce $F_2$ at chromatographic purities of 82.5 $\pm$ 1.3%. This is the first report of gram-scale production of $F_2$ from PPDGM using a novel ginsenoside-transforming $\beta$ -glucosidase of glycoside hydrolase family 1.-
dc.languageeng-
dc.publisher한국과학기술원-
dc.subjectbiotransformation-
dc.subjectginsenoside hydrolyzing glycosidase-
dc.subjectrecombinant enzyme-
dc.subjectginsenoside F2-
dc.subjectNovosphingobium aromaticivorans-
dc.subject생물전환-
dc.subject진세노사이드 글리코시드 가수분해 효소-
dc.subject재조합 효소-
dc.subject진세노사이드 $F_2$-
dc.titleEnzymatic biotransformation of ginsenosides $Rb_1$, $Rb_2$, Rc and Rd into $F_2$ by novel recombinant ginsenoside hydrolyzing glycosidase from Novosphingobium aromaticivorans-
dc.title.alternativeNovosphingobium aromaticivorans로부터의 새로운 재조합 가수분해 효소를 이용한 진세노사이드 $Rb_1$, $Rb_2$, Rc, Rd의 $F_2$ 로의 생물전환-
dc.typeThesis(Master)-
dc.identifier.CNRN325007-
dc.description.department한국과학기술원 :생명과학과,-
Appears in Collection
BS-Theses_Master(석사논문)
Files in This Item
There are no files associated with this item.

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0