Neuronal activation induced by femtosecond laser irradiation and its application on the cell-based assay = 펨토초 레이저를 이용한 신경세포 활성기술과 세포기반시험에 대한 응용

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Femtosecond laser stimulation on a neuron can generate localized reactive oxygen species (ROS) which can induce neuronal activation. However, the detailed mechanism of such neuronal activation method has not been elucidated, so the generation and propagation of calcium signal at the targeted neuron were investigated in this research. I found that superoxide is the main source of calcium elevation at the single cell level, while calcium propagation at the network level was blocked by extracellular calcium depletion. Such calcium elevation,propagation and cell death pattern due to laser stimulation were different when the neurons were treated with $A\beta$ , a protein accumulated in brains with Alzheimer’s disease. The results collectively indicate that femtosecond laser stimulation may distinguish abnormal neurons treated with toxic drugs and healthy neuron through investigation on laser-induced responses, which may be further applied for drug screening at clinical study.
Choi, Chulheeresearcher최철희researcher
Description
한국과학기술원 :바이오및뇌공학과,
Publisher
한국과학기술원
Issue Date
2016
Identifier
325007
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2016.8 ,[ii, 39 p. :]

Keywords

Neuron; Femtosecond laser; Reactive Oxygen Species; Calcium; Alzheimer's disease; 신경세포; 펨토초 레이저; 활성산소; 칼슘; 알츠하이머 병

URI
http://hdl.handle.net/10203/221432