DC Field | Value | Language |
---|---|---|
dc.contributor.author | Guo, Ya | ko |
dc.contributor.author | Xu, Quan | ko |
dc.contributor.author | Canzio, Daniele | ko |
dc.contributor.author | Shou, Jia | ko |
dc.contributor.author | Li, Jinhuan | ko |
dc.contributor.author | Gorkin, David U. | ko |
dc.contributor.author | Jung, Inkyung | ko |
dc.contributor.author | Wu, Haiyang | ko |
dc.contributor.author | Zhai, Yanan | ko |
dc.contributor.author | Tang, Yuanxiao | ko |
dc.contributor.author | Lu, Yichao | ko |
dc.contributor.author | Wu, Yonghu | ko |
dc.contributor.author | Jia, Zhilian | ko |
dc.contributor.author | Li, Wei | ko |
dc.contributor.author | Zhang, Michael Q. | ko |
dc.contributor.author | Ren, Bing | ko |
dc.contributor.author | Krainer, Adrian R. | ko |
dc.contributor.author | Maniatis, Tom | ko |
dc.contributor.author | Wu, Qiang | ko |
dc.date.accessioned | 2017-01-18T02:57:24Z | - |
dc.date.available | 2017-01-18T02:57:24Z | - |
dc.date.created | 2017-01-01 | - |
dc.date.created | 2017-01-01 | - |
dc.date.created | 2017-01-01 | - |
dc.date.created | 2017-01-01 | - |
dc.date.issued | 2015-08 | - |
dc.identifier.citation | CELL, v.162, no.4, pp.900 - 910 | - |
dc.identifier.issn | 0092-8674 | - |
dc.identifier.uri | http://hdl.handle.net/10203/219689 | - |
dc.description.abstract | CTCF and the associated cohesin complex play a central role in insulator function and higher-order chromatin organization of mammalian genomes. Recent studies identified a correlation between the orientation of CTCF-binding sites (CBSs) and chromatin loops. To test the functional significance of this observation, we combined CRISPR/Cas9-based genomic-DNA-fragment editing with chromosome-conformation-capture experiments to show that the location and relative orientations of CBSs determine the specificity of long-range chromatin looping in mammalian genomes, using protocadherin (Pcdh) and beta-globin as model genes. Inversion of CBS elements within the Pcdh enhancer reconfigures the topology of chromatin loops between the distal enhancer and target promoters and alters gene-expression patterns. Thus, although enhancers can function in an orientation-independent manner in reporter assays, in the native chromosome context, the orientation of at least some enhancers carrying CBSs can determine both the architecture of topological chromatin domains and enhancer/promoter specificity. These findings reveal how 3D chromosome architecture can be encoded by linear genome sequences. | - |
dc.language | English | - |
dc.publisher | CELL PRESS | - |
dc.title | CRISPR Inversion of CTCF Sites Alters Genome Topology and Enhancer/Promoter Function | - |
dc.type | Article | - |
dc.identifier.wosid | 000359741400021 | - |
dc.identifier.scopusid | 2-s2.0-84939246295 | - |
dc.type.rims | ART | - |
dc.citation.volume | 162 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 900 | - |
dc.citation.endingpage | 910 | - |
dc.citation.publicationname | CELL | - |
dc.identifier.doi | 10.1016/j.cell.2015.07.038 | - |
dc.contributor.localauthor | Jung, Inkyung | - |
dc.contributor.nonIdAuthor | Guo, Ya | - |
dc.contributor.nonIdAuthor | Xu, Quan | - |
dc.contributor.nonIdAuthor | Canzio, Daniele | - |
dc.contributor.nonIdAuthor | Shou, Jia | - |
dc.contributor.nonIdAuthor | Li, Jinhuan | - |
dc.contributor.nonIdAuthor | Gorkin, David U. | - |
dc.contributor.nonIdAuthor | Wu, Haiyang | - |
dc.contributor.nonIdAuthor | Zhai, Yanan | - |
dc.contributor.nonIdAuthor | Tang, Yuanxiao | - |
dc.contributor.nonIdAuthor | Lu, Yichao | - |
dc.contributor.nonIdAuthor | Wu, Yonghu | - |
dc.contributor.nonIdAuthor | Jia, Zhilian | - |
dc.contributor.nonIdAuthor | Li, Wei | - |
dc.contributor.nonIdAuthor | Zhang, Michael Q. | - |
dc.contributor.nonIdAuthor | Ren, Bing | - |
dc.contributor.nonIdAuthor | Krainer, Adrian R. | - |
dc.contributor.nonIdAuthor | Maniatis, Tom | - |
dc.contributor.nonIdAuthor | Wu, Qiang | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | PROTEIN-DNA INTERACTIONS | - |
dc.subject.keywordPlus | ALPHA GENE-CLUSTER | - |
dc.subject.keywordPlus | PROTOCADHERIN-ALPHA | - |
dc.subject.keywordPlus | GAMMA-PROTOCADHERINS | - |
dc.subject.keywordPlus | REGULATORY ELEMENTS | - |
dc.subject.keywordPlus | PROMOTER CHOICE | - |
dc.subject.keywordPlus | CELL-ADHESION | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | BETA | - |
dc.subject.keywordPlus | ORGANIZATION | - |
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