Combinatorial treatment with lithium chloride enhances recombinant antibody production in transiently transfected CHO and HEK293E cells

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dc.contributor.authorKim, Che Linko
dc.contributor.authorHa, Tae Kwangko
dc.contributor.authorLee, Gyun Minko
dc.date.accessioned2017-01-18T02:53:22Z-
dc.date.available2017-01-18T02:53:22Z-
dc.date.created2016-12-13-
dc.date.created2016-12-13-
dc.date.issued2016-09-
dc.identifier.citationBIOTECHNOLOGY AND BIOPROCESS ENGINEERING, v.21, no.5, pp.667 - 675-
dc.identifier.issn1226-8372-
dc.identifier.urihttp://hdl.handle.net/10203/219670-
dc.description.abstractLithium chloride (LiCl), which induces cell cycle arrest at G2/M phase, is known as a specific production rate (q (p))-enhancing additive in recombinant Chinese hamster ovary (CHO) cell culture. To determine the potential of LiCl as a chemical additive that enhances transient gene expression (TGE), LiCl was added to the CHO-NK and human embryonic kidney 293E (HEK293E) cell cultures before and/or after transfection with polyethylenimine as a transfection reagent. The effect of this addition on transfection efficiency (pre-treatment) and q (p) enhancement during TGE (post-treatment) was examined. For the TGE of monoclonal antibody (mAb) in CHO-NK cells, pretreatment alone with 10 mM LiCl and post-treatment alone with 5 mM LiCl resulted in 1.2- and 3.4-fold increase of maximum mAb concentration (MMC), respectively, compared with the TGE without LiCl treatment. Furthermore, combinatorial treatment with LiCl (10 mM for pre-treatment and 5 mM for post-treatment) synergistically increased the TGE of mAb (5.3-fold increase in MMC). Likewise, combinatorial treatment with LiCl (10 mM for pre-treatment and 15 mM for post-treatment) in HEK293E cells synergistically increased the TGE of mAb (4.9-fold increase in MMC). Taken together, the data obtained here demonstrate that combinatorial treatment with LiCl is a useful means to improve TGE in CHO as well as HEK293 cells.-
dc.languageEnglish-
dc.publisherKOREAN SOC BIOTECHNOLOGY & BIOENGINEERING-
dc.subjectHAMSTER OVARY CELLS-
dc.subjectINTACT YEAST-CELLS-
dc.subjectFC-FUSION PROTEIN-
dc.subjectGENE-EXPRESSION-
dc.subjectVALPROIC ACID-
dc.subjectCULTURE-
dc.subjectTRANSFORMATION-
dc.subjectEFFICIENCY-
dc.subjectAPOPTOSIS-
dc.subjectLINE-
dc.titleCombinatorial treatment with lithium chloride enhances recombinant antibody production in transiently transfected CHO and HEK293E cells-
dc.typeArticle-
dc.identifier.wosid000387681800013-
dc.identifier.scopusid2-s2.0-84994754076-
dc.type.rimsART-
dc.citation.volume21-
dc.citation.issue5-
dc.citation.beginningpage667-
dc.citation.endingpage675-
dc.citation.publicationnameBIOTECHNOLOGY AND BIOPROCESS ENGINEERING-
dc.identifier.doi10.1007/s12257-016-0434-8-
dc.identifier.kciidART002163278-
dc.contributor.localauthorLee, Gyun Min-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorcell cycle arrest-
dc.subject.keywordAuthorCHO cells-
dc.subject.keywordAuthorHEK293E cells-
dc.subject.keywordAuthorlithium chloride-
dc.subject.keywordAuthortransient gene expression-
dc.subject.keywordPlusHAMSTER OVARY CELLS-
dc.subject.keywordPlusINTACT YEAST-CELLS-
dc.subject.keywordPlusFC-FUSION PROTEIN-
dc.subject.keywordPlusGENE-EXPRESSION-
dc.subject.keywordPlusVALPROIC ACID-
dc.subject.keywordPlusCULTURE-
dc.subject.keywordPlusTRANSFORMATION-
dc.subject.keywordPlusEFFICIENCY-
dc.subject.keywordPlusAPOPTOSIS-
dc.subject.keywordPlusLINE-
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