Glycomic profiling of targeted serum haptoglobin for gastric cancer using nano LC/MS and LC/MS/MS

Cited 13 time in webofscience Cited 0 time in scopus
  • Hit : 333
  • Download : 0
DC FieldValueLanguage
dc.contributor.authorLee, Sung Hyeonko
dc.contributor.authorJeong, Seunghyupko
dc.contributor.authorLee, Juako
dc.contributor.authorYeo, In Seokko
dc.contributor.authorOh, Myung Jinko
dc.contributor.authorKim, Unyongko
dc.contributor.authorKim, Suminko
dc.contributor.authorKim, Su Heeko
dc.contributor.authorPark, Seung-Yeolko
dc.contributor.authorKim, Jae-Hanko
dc.contributor.authorPark, Se Hoonko
dc.contributor.authorKim, Jung Hoeko
dc.contributor.authorAn, Hyun Jooko
dc.date.accessioned2017-01-18T02:52:38Z-
dc.date.available2017-01-18T02:52:38Z-
dc.date.created2016-12-27-
dc.date.created2016-12-27-
dc.date.issued2016-09-
dc.identifier.citationMOLECULAR BIOSYSTEMS, v.12, no.12, pp.3611 - 3621-
dc.identifier.issn1742-206X-
dc.identifier.urihttp://hdl.handle.net/10203/219667-
dc.description.abstractGastric cancer has one of the highest cancer mortality rates worldwide, largely because of difficulties in early-stage detection. Aberrant glycosylation in serum proteins is associated with many human diseases including inflammation and various types of cancer. Serum-based global glycan profiling using mass spectrometry has been explored and has already led to several potential glycan markers for several disease states. However, localization of the aberrant glycosylation is desirable in order to improve the specificity and sensitivity for clinical use. Here, we combined protein-specific immunoaffinity purification, glycan release, and MS analysis to examine haptoglobin glycosylation of gastric cancer patients for glyco-markers. Age-and sex-matched 60 serum samples (30 cancer patients and 30 healthy controls) were used to profile and quantify haptoglobin N-glycans. A T-test based statistical analysis was performed to identify potential glyco-markers for gastric cancer. Interestingly, abundances of several tri-and tetra-antennary fucosylated N-glycans were increased in gastric cancer patients. Additionally, structural analysis via LC/MS/MS indicated that the fucosylated complex type N-glycans were primarily decorated with antenna fucose, which can be categorized as sialyl-Lea or sialyl-Lex type structures. This platform demonstrates quantitative, structure-specific profiling of haptoglobin glycosylation for the purposes of biomarker discovery for gastric cancer.-
dc.languageEnglish-
dc.publisherROYAL SOC CHEMISTRY-
dc.subjectREACTIVE ALPHA-FETOPROTEIN-
dc.subjectN-GLYCOSYLATION STATUS-
dc.subjectHEPATOCELLULAR-CARCINOMA-
dc.subjectPANCREATIC-CANCER-
dc.subjectPROTEIN GLYCOSYLATION-
dc.subjectBETA-HAPTOGLOBIN-
dc.subjectCOLON-CANCER-
dc.subjectFUCOSYLATED HAPTOGLOBIN-
dc.subjectBREAST-CANCER-
dc.subjectEXPRESSION-
dc.titleGlycomic profiling of targeted serum haptoglobin for gastric cancer using nano LC/MS and LC/MS/MS-
dc.typeArticle-
dc.identifier.wosid000388946800010-
dc.identifier.scopusid2-s2.0-84996835393-
dc.type.rimsART-
dc.citation.volume12-
dc.citation.issue12-
dc.citation.beginningpage3611-
dc.citation.endingpage3621-
dc.citation.publicationnameMOLECULAR BIOSYSTEMS-
dc.identifier.doi10.1039/c6mb00559d-
dc.contributor.localauthorKim, Jung Hoe-
dc.contributor.nonIdAuthorLee, Sung Hyeon-
dc.contributor.nonIdAuthorJeong, Seunghyup-
dc.contributor.nonIdAuthorLee, Jua-
dc.contributor.nonIdAuthorOh, Myung Jin-
dc.contributor.nonIdAuthorKim, Unyong-
dc.contributor.nonIdAuthorKim, Sumin-
dc.contributor.nonIdAuthorKim, Su Hee-
dc.contributor.nonIdAuthorPark, Seung-Yeol-
dc.contributor.nonIdAuthorKim, Jae-Han-
dc.contributor.nonIdAuthorPark, Se Hoon-
dc.contributor.nonIdAuthorAn, Hyun Joo-
dc.description.isOpenAccessN-
dc.type.journalArticleArticle-
dc.subject.keywordPlusREACTIVE ALPHA-FETOPROTEIN-
dc.subject.keywordPlusN-GLYCOSYLATION STATUS-
dc.subject.keywordPlusHEPATOCELLULAR-CARCINOMA-
dc.subject.keywordPlusPANCREATIC-CANCER-
dc.subject.keywordPlusPROTEIN GLYCOSYLATION-
dc.subject.keywordPlusBETA-HAPTOGLOBIN-
dc.subject.keywordPlusCOLON-CANCER-
dc.subject.keywordPlusFUCOSYLATED HAPTOGLOBIN-
dc.subject.keywordPlusBREAST-CANCER-
dc.subject.keywordPlusEXPRESSION-
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 13 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0