Cerebellar Shank2 Regulates Excitatory Synapse Density, Motor Coordination, and Specific Repetitive and Anxiety-Like Behaviors

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Shank2 is a multidomain scaffolding protein implicated in the structural and functional coordination of multiprotein complexes at excitatory postsynaptic sites as well as in psychiatric disorders, including autism spectrum disorders. While Shank2 is strongly expressed in the cerebellum, whether Shank2 regulates cerebellar excitatory synapses, or contributes to the behavioral abnormalities observed in Shank2(-/-) mice, remains unexplored. Here we show that Shank2(-/-) mice show reduced excitatory synapse density in cerebellar Purkinje cells in association with reduced levels of excitatory postsynaptic proteins, including GluD2 and PSD-93, and impaired motor coordination in the Erasmus test. Shank2 deletion restricted to Purkinje cells (Pcp2-Cre; Shank2(fl/fl) mice) leads to similar reductions in excitatory synapse density, synaptic protein levels, and motor coordination. Pcp2-Cre; Shank2(fl/fl) mice do not recapitulate autistic-like behaviors observed in Shank2(-/-) mice, such as social interaction deficits, altered ultrasonic vocalizations, repetitive behaviors, and hyperactivity. However, Pcp2-Cre; Shank2(fl/fl) mice display enhanced repetitive behavior in the hole-board test and anxiety-like behavior in the light-dark test, which are not observed in Shan2(-/-) mice. These results implicate Shank2 in the regulation of cerebellar excitatory synapse density, motor coordination, and specific repetitive and anxiety-like behaviors.
Publisher
SOC NEUROSCIENCE
Issue Date
2016-11
Language
English
Article Type
Article
Keywords

GLUTAMATE-RECEPTOR DELTA-2; AUTISM SPECTRUM DISORDERS; PARALLEL FIBER SYNAPSES; POSTSYNAPTIC DENSITY; MUTANT MICE; PURKINJE-CELLS; NEUROPSYCHIATRIC DISORDERS; DIFFERENTIAL EXPRESSION; SCAFFOLD PROTEINS; BINDING-PROTEIN

Citation

JOURNAL OF NEUROSCIENCE, v.36, no.48, pp.12129 - 12143

ISSN
0270-6474
DOI
10.1523/JNEUROSCI.1849-16.2016
URI
http://hdl.handle.net/10203/219631
Appears in Collection
BS-Journal Papers(저널논문)
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