Identification of a Highly Conserved Hypothetical Protein TON_0340 as a Probable Manganese-Dependent Phosphatase

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dc.contributor.authorSohn, Young Sikko
dc.contributor.authorLee, Seong Gyuko
dc.contributor.authorLee, Kwang-Hoonko
dc.contributor.authorKu, Bonsuko
dc.contributor.authorShine, Ho-Chulko
dc.contributor.authorCha, Sun-Shinko
dc.contributor.authorKim, Yeon-Gilko
dc.contributor.authorLee, Hyun Sookko
dc.contributor.authorKang, Sung-Gyunko
dc.contributor.authorOh, Byung-Hako
dc.date.accessioned2017-01-09T08:08:24Z-
dc.date.available2017-01-09T08:08:24Z-
dc.date.created2017-01-09-
dc.date.created2017-01-09-
dc.date.issued2016-12-
dc.identifier.citationPLOS ONE, v.11, no.12-
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10203/216130-
dc.description.abstractA hypothetical protein TON_0340 of a Thermococcus species is a protein conserved in a variety of organisms including human. Herein, we present four different crystal structures of TON_0340, leading to the identification of an active-site cavity harboring a metal-binding site composed of six invariant aspartate and glutamate residues that coordinate one to three metal ions. Biochemical and mutational analyses involving many phosphorous compounds show that TON_0340 is a Mn2+-dependent phosphatase. Mg2+ binds to TON_0340 less tightly and activates the phosphatase activity less efficiently than Mn2+. Whereas Ca2+ and Zn2+ are able to bind to the protein, they are unable to activate its enzymatic activity. Since the active-site cavity is small and largely composed of nearly invariant stretches of 11 or 13 amino acids, the physiological substrates of TON_0340 and its homologues are likely to be a small and the same molecule. The Mn2+-bound TON_0340 structure provides a canonical model for the ubiquitously present TON_0340 homologues and lays a strong foundation for the elucidation of their substrate and biological function.-
dc.languageEnglish-
dc.publisherPUBLIC LIBRARY SCIENCE-
dc.subjectIB RIBONUCLEOTIDE REDUCTASE-
dc.subjectN-OXYGENASE AURF-
dc.subjectCRYSTAL-STRUCTURE-
dc.subjectSEQUENCE-
dc.subjectDNA-
dc.titleIdentification of a Highly Conserved Hypothetical Protein TON_0340 as a Probable Manganese-Dependent Phosphatase-
dc.typeArticle-
dc.identifier.wosid000389482700189-
dc.identifier.scopusid2-s2.0-85000645675-
dc.type.rimsART-
dc.citation.volume11-
dc.citation.issue12-
dc.citation.publicationnamePLOS ONE-
dc.identifier.doi10.1371/journal.pone.0167549-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorOh, Byung-Ha-
dc.contributor.nonIdAuthorLee, Kwang-Hoon-
dc.contributor.nonIdAuthorKu, Bonsu-
dc.contributor.nonIdAuthorShine, Ho-Chul-
dc.contributor.nonIdAuthorCha, Sun-Shin-
dc.contributor.nonIdAuthorKim, Yeon-Gil-
dc.contributor.nonIdAuthorLee, Hyun Sook-
dc.contributor.nonIdAuthorKang, Sung-Gyun-
dc.description.isOpenAccessY-
dc.type.journalArticleArticle-
dc.subject.keywordPlusIB RIBONUCLEOTIDE REDUCTASE-
dc.subject.keywordPlusN-OXYGENASE AURF-
dc.subject.keywordPlusCRYSTAL-STRUCTURE-
dc.subject.keywordPlusSEQUENCE-
dc.subject.keywordPlusDNA-
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