DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sohn, Jong Woo | ko |
dc.contributor.author | Oh, Youjin | ko |
dc.contributor.author | Kim, Ki Woo | ko |
dc.contributor.author | Lee, Syann | ko |
dc.contributor.author | Williams, Kevin W. | ko |
dc.contributor.author | Elmquist, Joel K. | ko |
dc.date.accessioned | 2016-12-14T02:25:55Z | - |
dc.date.available | 2022-06-02T21:00:53Z | - |
dc.date.created | 2016-12-05 | - |
dc.date.created | 2016-12-05 | - |
dc.date.issued | 2016-08 | - |
dc.identifier.citation | MOLECULAR METABOLISM, v.5, no.8, pp.669 - 679 | - |
dc.identifier.issn | 2212-8778 | - |
dc.identifier.uri | http://hdl.handle.net/10203/214851 | - |
dc.description.abstract | Objective: The ventromedial hypothalamic nucleus (VMH) regulates energy balance and glucose homeostasis. Leptin and insulin exert metabolic effects via their cognate receptors expressed by the steroidogenic factor 1 (SF1) neurons within the VMH. However, detailed cellular mechanisms involved in the regulation of these neurons by leptin and insulin remain to be identified. Methods: We utilized genetically-modified mouse models and performed patch-clamp electrophysiology experiments to resolve this issue. Results: We identified distinct populations of leptin-activated and leptin-inhibited SF1 neurons. In contrast, insulin uniformly inhibited SF1 neurons. Notably, we found that leptin-activated, leptin-inhibited, and insulin-inhibited SF1 neurons are distinct subpopulations within the VMH. Leptin depolarization of SF1 neuron also required the PI3K p110 beta catalytic subunit. This effect was mediated by the putative transient receptor potential C (TRPC) channel. On the other hand, hyperpolarizing responses of SF1 neurons by leptin and insulin required either of the p110 alpha or p110 beta catalytic subunits, and were mediated by the putative ATP-sensitive K+ (K-ATP) channel. Conclusions: Our results demonstrate that specific PI3K catalytic subunits are responsible for the acute effects of leptin and insulin on VMH SF1 neurons, and provide insights into the cellular mechanisms of leptin and insulin action on VMH SF1 neurons that regulate energy balance and glucose homeostasis. (C) 2016 The Authors. Published by Elsevier GmbH. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.subject | PHOSPHOINOSITIDE 3-KINASE PATHWAY | - |
dc.subject | VENTRAL PREMAMMILLARY NUCLEUS | - |
dc.subject | PROOPIOMELANOCORTIN NEURONS | - |
dc.subject | STEROIDOGENIC FACTOR-1 | - |
dc.subject | GLUCOSE-HOMEOSTASIS | - |
dc.subject | ENERGY HOMEOSTASIS | - |
dc.subject | RAPID INHIBITION | - |
dc.subject | TRPC CHANNELS | - |
dc.subject | MICE LACKING | - |
dc.subject | OBESITY | - |
dc.title | Leptin and insulin engage specific PI3K subunits in hypothalamic SF1 neurons | - |
dc.type | Article | - |
dc.identifier.wosid | 000386882400008 | - |
dc.identifier.scopusid | 2-s2.0-84989877542 | - |
dc.type.rims | ART | - |
dc.citation.volume | 5 | - |
dc.citation.issue | 8 | - |
dc.citation.beginningpage | 669 | - |
dc.citation.endingpage | 679 | - |
dc.citation.publicationname | MOLECULAR METABOLISM | - |
dc.identifier.doi | 10.1016/j.molmet.2016.06.004 | - |
dc.embargo.terms | 2017-04-02 | - |
dc.contributor.localauthor | Sohn, Jong Woo | - |
dc.contributor.nonIdAuthor | Kim, Ki Woo | - |
dc.contributor.nonIdAuthor | Lee, Syann | - |
dc.contributor.nonIdAuthor | Williams, Kevin W. | - |
dc.contributor.nonIdAuthor | Elmquist, Joel K. | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Cellular mechanism | - |
dc.subject.keywordAuthor | Conditional knockout mouse | - |
dc.subject.keywordAuthor | Patch clamp technique | - |
dc.subject.keywordAuthor | Functional heterogeneity | - |
dc.subject.keywordAuthor | Homeostasis | - |
dc.subject.keywordPlus | PHOSPHOINOSITIDE 3-KINASE PATHWAY | - |
dc.subject.keywordPlus | VENTRAL PREMAMMILLARY NUCLEUS | - |
dc.subject.keywordPlus | PROOPIOMELANOCORTIN NEURONS | - |
dc.subject.keywordPlus | STEROIDOGENIC FACTOR-1 | - |
dc.subject.keywordPlus | GLUCOSE-HOMEOSTASIS | - |
dc.subject.keywordPlus | ENERGY HOMEOSTASIS | - |
dc.subject.keywordPlus | RAPID INHIBITION | - |
dc.subject.keywordPlus | TRPC CHANNELS | - |
dc.subject.keywordPlus | MICE LACKING | - |
dc.subject.keywordPlus | OBESITY | - |
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