Holographic intravital microscopy for 2-D and 3-D imaging intact circulating blood cells in microcapillaries of live mice

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Intravital microscopy is an essential tool that reveals behaviours of live cells under conditions close to natural physiological states. So far, although various approaches for imaging cells in vivo have been proposed, most require the use of labelling and also provide only qualitative imaging information. Holographic imaging approach based on measuring the refractive index distributions of cells, however, circumvent these problems and offer quantitative and label-free imaging capability. Here, we demonstrate in vivo two- and three-dimensional holographic imaging of circulating blood cells in intact microcapillaries of live mice. The measured refractive index distributions of blood cells provide morphological and biochemical properties including three-dimensional cell shape, haemoglobin concentration, and haemoglobin contents at the individual cell level. With the present method, alterations in blood flow dynamics in live healthy and sepsis-model mice were also investigated
Publisher
NATURE PUBLISHING GROUP
Issue Date
2016-09
Language
English
Article Type
Article
Citation

SCIENTIFIC REPORTS, v.6, pp.33084

ISSN
2045-2322
DOI
10.1038/srep33084
URI
http://hdl.handle.net/10203/213209
Appears in Collection
NT-Journal Papers(저널논문)PH-Journal Papers(저널논문)
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