DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ai, Minrong | ko |
dc.contributor.author | Blais, Steven | ko |
dc.contributor.author | Park, Jin-Yong | ko |
dc.contributor.author | Min, Soohong | ko |
dc.contributor.author | Neubert, Thomas A. | ko |
dc.contributor.author | Suh, Greg S. B. | ko |
dc.date.accessioned | 2016-07-25T08:30:14Z | - |
dc.date.available | 2016-07-25T08:30:14Z | - |
dc.date.created | 2016-07-12 | - |
dc.date.created | 2016-07-12 | - |
dc.date.issued | 2013-06 | - |
dc.identifier.citation | JOURNAL OF NEUROSCIENCE, v.33, no.26, pp.10741 - 10749 | - |
dc.identifier.issn | 0270-6474 | - |
dc.identifier.uri | http://hdl.handle.net/10203/211905 | - |
dc.description.abstract | Drosophila olfactory sensory neurons express either odorant receptors or ionotropic glutamate receptors (IRs). The sensory neurons that express IR64a, a member of the IR family, send axonal projections to either the DC4 or DP1m glomeruli in the antennal lobe. DC4 neurons respond specifically to acids/protons, whereas DP1m neurons respond to a broad spectrum of odorants. The molecular composition of IR64a-containing receptor complexes in either DC4 or DP1m neurons is not known, however. Here, we immunoprecipitated the IR64a protein from lysates of fly antennal tissue and identified IR8a as a receptor subunit physically associated with IR64a by mass spectrometry. IR8a mutants and flies in which IR8a was knocked down by RNAi in IR64a+ neurons exhibited defects in acid-evoked physiological and behavioral responses. Furthermore, we found that the loss of IR8a caused a significant reduction in IR64a protein levels. When expressed in Xenopus oocytes, IR64a and IR8a formed a functional ion channel that allowed ligand-evoked cation currents. These findings provide direct evidence that IR8a is a subunit that forms a functional olfactory receptor with IR64a in vivo to mediate odor detection | - |
dc.language | English | - |
dc.publisher | SOC NEUROSCIENCE | - |
dc.subject | CHEMOSENSORY RECEPTORS | - |
dc.subject | OLFACTORY SYSTEM | - |
dc.subject | G-PROTEINS | - |
dc.subject | ORGANIZATION | - |
dc.subject | ARCHITECTURE | - |
dc.subject | SENSILLA | - |
dc.subject | NEURONS | - |
dc.subject | ANTENNA | - |
dc.subject | REPRESENTATION | - |
dc.subject | MELANOGASTER | - |
dc.title | Ionotropic Glutamate Receptors IR64a and IR8a Form a Functional Odorant Receptor Complex In Vivo in Drosophila | - |
dc.type | Article | - |
dc.identifier.wosid | 000320928900018 | - |
dc.identifier.scopusid | 2-s2.0-84879305528 | - |
dc.type.rims | ART | - |
dc.citation.volume | 33 | - |
dc.citation.issue | 26 | - |
dc.citation.beginningpage | 10741 | - |
dc.citation.endingpage | 10749 | - |
dc.citation.publicationname | JOURNAL OF NEUROSCIENCE | - |
dc.identifier.doi | 10.1523/JNEUROSCI.5419-12.2013 | - |
dc.contributor.localauthor | Suh, Greg S. B. | - |
dc.contributor.nonIdAuthor | Ai, Minrong | - |
dc.contributor.nonIdAuthor | Blais, Steven | - |
dc.contributor.nonIdAuthor | Park, Jin-Yong | - |
dc.contributor.nonIdAuthor | Min, Soohong | - |
dc.contributor.nonIdAuthor | Neubert, Thomas A. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CHEMOSENSORY RECEPTORS | - |
dc.subject.keywordPlus | OLFACTORY SYSTEM | - |
dc.subject.keywordPlus | G-PROTEINS | - |
dc.subject.keywordPlus | ORGANIZATION | - |
dc.subject.keywordPlus | ARCHITECTURE | - |
dc.subject.keywordPlus | SENSILLA | - |
dc.subject.keywordPlus | NEURONS | - |
dc.subject.keywordPlus | ANTENNA | - |
dc.subject.keywordPlus | REPRESENTATION | - |
dc.subject.keywordPlus | MELANOGASTER | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.