DC Field | Value | Language |
---|---|---|
dc.contributor.author | Ai, Minrong | ko |
dc.contributor.author | Mills, Holly | ko |
dc.contributor.author | Kanai, Makoto | ko |
dc.contributor.author | Lai, Jason | ko |
dc.contributor.author | Deng, Jingjing | ko |
dc.contributor.author | Schreiter, Eric | ko |
dc.contributor.author | Looger, Loren | ko |
dc.contributor.author | Neubert, Thomas | ko |
dc.contributor.author | Suh, Greg | ko |
dc.date.accessioned | 2016-07-25T08:27:36Z | - |
dc.date.available | 2016-07-25T08:27:36Z | - |
dc.date.created | 2016-07-12 | - |
dc.date.created | 2016-07-12 | - |
dc.date.created | 2016-07-12 | - |
dc.date.issued | 2015-09 | - |
dc.identifier.citation | PLOS ONE, v.10, no.9 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/10203/211878 | - |
dc.description.abstract | Genetically encoded calcium indicators (GECIs) permit imaging intracellular calcium transients. Among GECIs, the GFP-based GCaMPs are the most widely used because of their high sensitivity and rapid response to changes in intracellular calcium concentrations. Here we report that the fluorescence of GCaMPs-including GCaMP3, GCaMP5 and GCaMP6-can be converted from green to red following exposure to blue-green light (450500 nm). This photoconversion occurs in both insect and mammalian cells and is enhanced in a low oxygen environment. The red fluorescent GCaMPs retained calcium responsiveness, albeit with reduced sensitivity. We identified several amino acid residues in GCaMP important for photoconversion and generated a GCaMP variant with increased photoconversion efficiency in cell culture. This light-induced spectral shift allows the ready labeling of specific, targeted sets of GCaMP-expressing cells for functional imaging in the red channel. Together, these findings indicate the potential for greater utility of existing GCaMP reagents, including transgenic animals | - |
dc.language | English | - |
dc.publisher | PUBLIC LIBRARY SCIENCE | - |
dc.subject | ENCODED CALCIUM INDICATORS | - |
dc.subject | FLUORESCENT PROTEIN | - |
dc.subject | NEURAL ACTIVITY | - |
dc.subject | CA2+ INDICATORS | - |
dc.subject | PHOTOACTIVATION | - |
dc.subject | CONVERSION | - |
dc.subject | MECHANISM | - |
dc.subject | TRACKING | - |
dc.subject | LIGHT | - |
dc.subject | CELL | - |
dc.title | Green-to-Red Photoconversion of GCaMP | - |
dc.type | Article | - |
dc.identifier.wosid | 000361790200060 | - |
dc.identifier.scopusid | 2-s2.0-84946594010 | - |
dc.type.rims | ART | - |
dc.citation.volume | 10 | - |
dc.citation.issue | 9 | - |
dc.citation.publicationname | PLOS ONE | - |
dc.identifier.doi | 10.1371/journal.pone.0138127 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Suh, Greg | - |
dc.contributor.nonIdAuthor | Ai, Minrong | - |
dc.contributor.nonIdAuthor | Mills, Holly | - |
dc.contributor.nonIdAuthor | Kanai, Makoto | - |
dc.contributor.nonIdAuthor | Lai, Jason | - |
dc.contributor.nonIdAuthor | Deng, Jingjing | - |
dc.contributor.nonIdAuthor | Schreiter, Eric | - |
dc.contributor.nonIdAuthor | Looger, Loren | - |
dc.contributor.nonIdAuthor | Neubert, Thomas | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | ENCODED CALCIUM INDICATORS | - |
dc.subject.keywordPlus | FLUORESCENT PROTEIN | - |
dc.subject.keywordPlus | NEURAL ACTIVITY | - |
dc.subject.keywordPlus | CA2+ INDICATORS | - |
dc.subject.keywordPlus | PHOTOACTIVATION | - |
dc.subject.keywordPlus | CONVERSION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | TRACKING | - |
dc.subject.keywordPlus | LIGHT | - |
dc.subject.keywordPlus | CELL | - |
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