박테리오로돕신의 생산을 위한 Halobacterium halobium R1의 배양시 글리세롤 및 배양 조건의 영향The Effects of Glycerol and Growth Conditions on the Production of Bacteriorhodopsin by Halobacterium halobium R1

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Bacteriorhodopsin is a membrane bound protein of halobacterium, which has a potential to be used for constructing molecular electron device and optical computers. In this study, the effects of glycerol and growth conditions on the production of bacterorhodopsin by Halobacterium halobium R1 were examined. Glycerol stimulated cell growth, and the final cell concentration increased with increasing glycerol concentration. The pH of the culture increased from 7 to 8 during growth. Varying the initial pH between 6 and 8 had little effect on the cell growth. When the dissolved oxygen level was high, cell growth was enhanced but the percentage of bacteriorhodopsin was lower (1.25%) than that (2.1%) under low dissolved oxygen level. It was concluded that growing cells to a high concentration prior to inducing bacteriorhodopsin formation was important to increase bacteriorhodopsin productivity.
Publisher
한국생물공학회
Issue Date
1997-07
Language
Korean
Citation

KSBB JOURNAL, v.12, no.3, pp.309 - 313

ISSN
1225-7117
URI
http://hdl.handle.net/10203/21012
Appears in Collection
CBE-Journal Papers(저널논문)
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