Simple synthesis of functionalized superparamagnetic magnetite/silica core/shell nanoparticles and their application as magnetically separable high-performance biocatalysts

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Uniformly sized silica-coated magnetic nanoparticles (magnetite@silica) are synthesized in a simple one-pot process using reverse micelles as nanoreactors. The core diameter of the magnetic nanoparticles is easily controlled by adjusting the w value ([polar solvent]/[surfactant]) in the reverse-micelle solution, and the thickness of the silica shell is easily controlled by varying the amount of tetraethyl orthosilicate added after the synthesis of the magnetite cores. Several grams of monodisperse magnetite@silica nanoparticles can be synthesized without going through any size-selection process. When crosslinked enzyme molecules form clusters on the surfaces of the magnetite@silica nanoparticles, the resulting hybrid composites are magnetically separable, highly active, and stable under harsh shaking conditions for more than 15 days. Conversely, covalently attached enzymes on the surface of the magnetite@silica nanoparticles are deactivated under the same conditions.
Publisher
WILEY-V C H VERLAG GMBH
Issue Date
2008-01
Language
English
Article Type
Article
Keywords

HISTIDINE-TAGGED PROTEINS; MESOPOROUS SILICA; COBALT NANORODS; NANOCRYSTALS; NANOCOMPOSITES; SIZE; CRYSTALLINE; SEPARATION; SHAPE; WATER

Citation

SMALL, v.4, no.1, pp.143 - 152

ISSN
1613-6810
DOI
10.1002/smll.200700456
URI
http://hdl.handle.net/10203/20958
Appears in Collection
CBE-Journal Papers(저널논문)
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