Modular Optimization of a Hemicellulose-Utilizing Pathway in Corynebacterium glutamicum for Consolidated Bioprocessing of Hemicellulosic Biomass

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Hemicellulose, which is the second most abundant polysaccharide in nature after cellulose, has the potential to become a major feedstock for microbial fermentation to produce various biofuels and chemicals. To utilize hemicellulose economically, it is necessary to develop a consolidated bioprocess (CBP), in which all processes from biomass degradation to the production of target products occur in a single bioreactor. Here, we report a modularly engineered Corynebacterium glutamicum strain suitable for CBP using hemicellulosic biomass (xylan) as a feedstock. The hemicellulose-utilizing pathway was divided into three distinct modules, and each module was separately optimized. In the module for xylose utilization, the expression level of the xylose isomerase (xylA) and xylulokinase (xylB) genes was optimized with synthetic promoters of different strengths. Then, the module for xylose transport was engineered with combinatorial sets of synthetic promoters and heterologous transporters to achieve the fastest cell growth rate on xylose (0.372 h(-1)). Next, the module for the enzymatic degradation of xylan to xylose was also engineered with different combinations of promoters and signal peptides to efficiently secrete both endoxylanase and xylosidase into the extracellular medium. Finally, each optimized module was integrated into a single plasmid to construct a highly efficient xylan-utilizing pathway. Subsequently, the direct production of lysine from xylan was successfully demonstrated with the engineered pathway. To the best of our knowledge, this is the first report of the development of a consolidated bioprocessing C. glutamicum strain for hemicellulosic biomass
Publisher
AMER CHEMICAL SOC
Issue Date
2016-04
Language
English
Article Type
Article
Keywords

ETHANOL-PRODUCTION; ESCHERICHIA-COLI; BETA-XYLOSIDASE; SACCHAROMYCES-CEREVISIAE; LIGNOCELLULOSIC BIOMASS; SUCCINATE PRODUCTION; SECRETORY PRODUCTION; LYSINE PRODUCTION; BACILLUS-PUMILUS; GENE-EXPRESSION

Citation

ACS SYNTHETIC BIOLOGY, v.5, no.4, pp.334 - 343

ISSN
2161-5063
DOI
10.1021/acssynbio.5b00228
URI
http://hdl.handle.net/10203/209530
Appears in Collection
CBE-Journal Papers(저널논문)
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