Production of poly(3-hydroxybutyrate) from whey by fed-batch culture of recombinant Escherichia coli in a pilot-scale fermenter

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Production of poly(3-hydroxybutyrate) [P(3HB)] from whey by fed-batch culture of recombinant Escherichia coli CGSC 4401 harboring a plasmid containing the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes was examined in a 30 l fermenter supplying air only. With lactose below 2 g l(-1), cells grew to 12 g dry cell l(-1) with 9% (w/w) P(3HB) content. Accumulation of P(3HB) could be triggered by increasing lactose to 20 g l(-1). By employing this strategy, 51 g dry cell l(-1) was obtained with a 70% (w/w) P(3HB) content after 26 h. The productivity was 1.35 g P(3HB) l(-1) h(-1). The same fermentation strategy was used in a 300 l fermenter, and 30 g dry cell l(-1) with 67% (w/w) P(3HB) content was obtained in 20 h.
Publisher
KLUWER ACADEMIC PUBL
Issue Date
2002-02
Language
English
Article Type
Article
Keywords

POLYHYDROXYALKANOATE PRODUCTION

Citation

BIOTECHNOLOGY LETTERS, v.24, no.3, pp.185 - 189

ISSN
0141-5492
URI
http://hdl.handle.net/10203/20861
Appears in Collection
CBE-Journal Papers(저널논문)
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