Constructive remodeling of a synthetic endothelial extracellular matrix

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The construction of well-controllable in vitro models of physiological and pathological vascular endothelium remains a fundamental challenge in tissue engineering and drug development. Here, we present an approach for forming a synthetic endothelial extracellular matrix (ECM) that closely resembles that of the native structure by locally depositing basement membrane materials onto type 1 collagen nanofibers only in a region adjacent to the endothelial cell (EC) monolayer. Culturing the EC monolayer on this synthetic endothelial ECM remarkably enhanced its physiological properties, reducing its vascular permeability, and promoting a stabilized, quiescent phenotype. We demonstrated that the EC monolayer on the synthetic endothelial ECM neither creates non-physiological barriers to cell-cell or cell-ECM interactions, nor hinders molecular diffusion of growth factors and other molecules. The synthetic endothelial ECM and vascular endothelium on it may help us enter in a new phase of research in which various models of the biological barrier behavior can be tested experimentally.
Publisher
NATURE PUBLISHING GROUP
Issue Date
2015-12
Language
English
Article Type
Article
Keywords

BLOOD-BRAIN-BARRIER; IN-VITRO; ASSAY; PERMEABILITY; MODEL; MORPHOGENESIS; TRANSPORT; HEALTH

Citation

SCIENTIFIC REPORTS, v.5

ISSN
2045-2322
DOI
10.1038/srep18290
URI
http://hdl.handle.net/10203/207813
Appears in Collection
ME-Journal Papers(저널논문)
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