DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bang, Hyun Bae | ko |
dc.contributor.author | Lee, Yoon Hyeok | ko |
dc.contributor.author | Kim, Sun Chang | ko |
dc.contributor.author | Sung, Chang Keun | ko |
dc.contributor.author | Jeong, Ki Jun | ko |
dc.date.accessioned | 2016-06-07T09:02:26Z | - |
dc.date.available | 2016-06-07T09:02:26Z | - |
dc.date.created | 2016-02-15 | - |
dc.date.created | 2016-02-15 | - |
dc.date.created | 2016-02-15 | - |
dc.date.created | 2016-02-15 | - |
dc.date.issued | 2016-01 | - |
dc.identifier.citation | MICROBIAL CELL FACTORIES, v.15 | - |
dc.identifier.issn | 1475-2859 | - |
dc.identifier.uri | http://hdl.handle.net/10203/207702 | - |
dc.description.abstract | Background: Plant parasitic nematodes are harmful to agricultural crops and plants, and may cause severe yield losses. Cinnamaldehyde, a volatile, yellow liquid commonly used as a flavoring or food additive, is increasingly becoming a popular natural nematicide because of its high nematicidal activity and, there is a high demand for the development of a biological platform to produce cinnamaldehyde. Results: We engineered Escherichia coli as an eco-friendly biological platform for the production of cinnamaldehyde. In E. coli, cinnamaldehyde can be synthesized from intracellular L-phenylalanine, which requires the activities of three enzymes: phenylalanine-ammonia lyase (PAL), 4-coumarate: CoA ligase (4CL), and cinnamoyl-CoA reductase (CCR). For the efficient production of cinnamaldehyde in E. coli, we first examined the activities of enzymes from different sources and a gene expression system for the selected enzymes was constructed. Next, the metabolic pathway for L-phenylalanine biosynthesis was engineered to increase the intracellular pool of L-phenylalanine, which is a main precursor of cinnamaldehyde. Finally, we tried to produce cinnamaldehyde with the engineered E. coli. According to this result, cinnamaldehyde production as high as 75 mg/L could be achieved, which was about 35-fold higher compared with that in the parental E. coli W3110 harboring a plasmid for cinnamaldehyde biosynthesis. We also confirmed that cinnamaldehyde produced by our engineered E. coli had a nematicidal activity similar to the activity of commercial cinnamaldehyde by nematicidal assays against Bursaphelenchus xylophilus. Conclusion: As a potential natural pesticide, cinnamaldehyde was successfully produced in E. coli by construction of the biosynthesis pathway and, its production titer was also significantly increased by engineering the metabolic pathway of L-phenylalanine. | - |
dc.language | English | - |
dc.publisher | BIOMED CENTRAL LTD | - |
dc.title | Metabolic engineering of Escherichia coli for the production of cinnamaldehyde | - |
dc.type | Article | - |
dc.identifier.wosid | 000368481500002 | - |
dc.identifier.scopusid | 2-s2.0-84955267195 | - |
dc.type.rims | ART | - |
dc.citation.volume | 15 | - |
dc.citation.publicationname | MICROBIAL CELL FACTORIES | - |
dc.identifier.doi | 10.1186/s12934-016-0415-9 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Kim, Sun Chang | - |
dc.contributor.localauthor | Jeong, Ki Jun | - |
dc.contributor.nonIdAuthor | Lee, Yoon Hyeok | - |
dc.contributor.nonIdAuthor | Sung, Chang Keun | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Escherichia coli | - |
dc.subject.keywordAuthor | Cinnamaldehyde | - |
dc.subject.keywordAuthor | L-Phenylalanine | - |
dc.subject.keywordAuthor | Metabolic engineering | - |
dc.subject.keywordAuthor | Nematodes | - |
dc.subject.keywordPlus | PHENYLALANINE AMMONIA-LYASE | - |
dc.subject.keywordPlus | PLANT-PARASITIC NEMATODES | - |
dc.subject.keywordPlus | P-HYDROXYCINNAMIC ACID | - |
dc.subject.keywordPlus | ROOT-KNOT NEMATODES | - |
dc.subject.keywordPlus | COENZYME-A LIGASE | - |
dc.subject.keywordPlus | ARABIDOPSIS-THALIANA | - |
dc.subject.keywordPlus | PHOSPHOTRANSFERASE SYSTEM | - |
dc.subject.keywordPlus | PREPHENATE DEHYDRATASE | - |
dc.subject.keywordPlus | MELOIDOGYNE-INCOGNITA | - |
dc.subject.keywordPlus | CINNAMOYL-COA | - |
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