DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Hee Yeon | ko |
dc.contributor.author | Lee, Joong-Jae | ko |
dc.contributor.author | Kim, Nury | ko |
dc.contributor.author | Heo, Won Do | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2016-06-07T08:56:27Z | - |
dc.date.available | 2016-06-07T08:56:27Z | - |
dc.date.created | 2016-03-14 | - |
dc.date.created | 2016-03-14 | - |
dc.date.created | 2016-03-14 | - |
dc.date.issued | 2016-02 | - |
dc.identifier.citation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.470, no.4, pp.857 - 863 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | http://hdl.handle.net/10203/207634 | - |
dc.description.abstract | Probing protein-protein interactions in living cells is crucial for understanding the protein functions and developing drugs. Small-sized protein binders are considered effective and useful for such analysis. Here we describe the development and use of a repebody, which is a protein binder composed of LRR (Leucine-rich repeat) modules, for tracking protein-protein interaction and localization in real-time through live-cell imaging. A repebody with high affinity for a red fluorescent protein was selected through a phage display, fused with a green fluorescent protein, and applied for tracing a red fluorescent protein-fused target protein in mammalian cells. The potential and utility of our approach was demonstrated by tracking the rapamycin-mediated interaction between FKBP12-rapamycin binding (FRB) domain and a FK506-binding protein (FKBP) and their localization by live-cell imaging. The present approach can be widely used for the analysis of protein-protein interaction and an understanding of complex biological processes in living cells. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Tracking protein-protein interaction and localization in living cells using a high-affinity molecular binder | - |
dc.type | Article | - |
dc.identifier.wosid | 000370582300013 | - |
dc.identifier.scopusid | 2-s2.0-84957548808 | - |
dc.type.rims | ART | - |
dc.citation.volume | 470 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 857 | - |
dc.citation.endingpage | 863 | - |
dc.citation.publicationname | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.identifier.doi | 10.1016/j.bbrc.2016.01.129 | - |
dc.contributor.localauthor | Heo, Won Do | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Protein-protein interactions | - |
dc.subject.keywordAuthor | Red fluorescent protein | - |
dc.subject.keywordAuthor | Intracellular antibody | - |
dc.subject.keywordAuthor | Colocalization analysis | - |
dc.subject.keywordAuthor | Live-cell imaging | - |
dc.subject.keywordPlus | NANOBODIES | - |
dc.subject.keywordPlus | FRAGMENTS | - |
dc.subject.keywordPlus | DISPLAY | - |
dc.subject.keywordPlus | DESIGN | - |
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