DC Field | Value | Language |
---|---|---|
dc.contributor.author | Yim, SC | ko |
dc.contributor.author | Jeong, Kijun | ko |
dc.contributor.author | Chang, Ho Nam | ko |
dc.contributor.author | Lee, SangYup | ko |
dc.date.accessioned | 2010-12-02T07:58:11Z | - |
dc.date.available | 2010-12-02T07:58:11Z | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.created | 2012-02-06 | - |
dc.date.issued | 2001-11 | - |
dc.identifier.citation | BIOPROCESS AND BIOSYSTEMS ENGINEERING, v.24, no.4, pp.249 - 254 | - |
dc.identifier.issn | 1615-7591 | - |
dc.identifier.uri | http://hdl.handle.net/10203/20657 | - |
dc.description.abstract | Secretory production of human granulocyte colony-stimulating factor fusion protein (hG-CSF) by fed-batch culture of Escherichia coli was investigated in both 2.5-L and 30-L fermentors, To develop a fed-batch culture condition that allows efficient production of hG-CSF, different feeding strategies including pH-stat, exponential and constant feeding were examined. Among these, the constant feeding strategy (0.228 g glucosexmin(-1)) and the exponential feeding that supports a low specific growth rate (mu=0.116 h(-1)) resulted in the best hG-CSF production. Under these conditions, 4.4 gxL(-1) of hG-CSF was produced. The effect of induction time on the protein production was also investigated. For the fed-batch cultures carried out with the pH-stat and exponential feeding strategies, induction at higher cell density (late-exponential phase) resulted in more hG-CSF production compared with induction at lower cell density (early to mid-exponential phase). The constant feeding strategy that supported best hG-CSF production was applied to the scale-up production of hG-CSF in 30 L of fermentor. The maximum dry cell weight and hG-CSF concentration of 51.7 and 4.2 gxL(-1), respectively, was obtained. | - |
dc.language | English | - |
dc.language.iso | en_US | en |
dc.publisher | SPRINGER-VERLAG | - |
dc.title | High-level secretory production of human granulocyte-colony stimulating factor by fed-batch culture of recombinant Escherichia coli | - |
dc.type | Article | - |
dc.identifier.wosid | 000173133500006 | - |
dc.identifier.scopusid | 2-s2.0-0035660851 | - |
dc.type.rims | ART | - |
dc.citation.volume | 24 | - |
dc.citation.issue | 4 | - |
dc.citation.beginningpage | 249 | - |
dc.citation.endingpage | 254 | - |
dc.citation.publicationname | BIOPROCESS AND BIOSYSTEMS ENGINEERING | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Jeong, Kijun | - |
dc.contributor.localauthor | Chang, Ho Nam | - |
dc.contributor.localauthor | Lee, SangYup | - |
dc.contributor.nonIdAuthor | Yim, SC | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | CELL-DENSITY CULTURE | - |
dc.subject.keywordPlus | PURIFICATION | - |
dc.subject.keywordPlus | FERMENTATION | - |
dc.subject.keywordPlus | CULTIVATION | - |
dc.subject.keywordPlus | METABOLISM | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | PROTEIN | - |
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