Production of poly(3-hydroxybutyrate) from whey by cell recycle fed-batch culture of recombinant Escherichia coli

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A new fermentation strategy using cell recycle membrane system was developed for the efficient production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes. By cell recycle, fed-batch cultivation employing an external membrane module, the working volume of fermentation could be constantly maintained at 2.3 l. The final cell concentration, PHB concentration and PHB content of 194 g l(-1), 168 g l(-1) and 87%, respectively, were obtained in 36.5 h by the pH-stat cell recycle fed-batch culture using whey solution concentrated to contain 280 g lactose l(-1) as a feeding solution, resulting in a high productivity of 4.6 g PHB l(-1) h(-1).
Publisher
SPRINGER
Issue Date
2001-02
Language
English
Article Type
Article
Keywords

BACTERIAL POLYHYDROXYALKANOATES

Citation

BIOTECHNOLOGY LETTERS, v.23, no.3, pp.235 - 240

ISSN
0141-5492
URI
http://hdl.handle.net/10203/20652
Appears in Collection
CBE-Journal Papers(저널논문)
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