Aminovalerate and Glutarate Production Pathway Introduced into Escherichia coli

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dc.contributor.authorKo, Yoo-Sungko
dc.contributor.authorPark, Si Jaeko
dc.contributor.authorKim, Eun Youngko
dc.contributor.authorNoh, Wonko
dc.contributor.authorPark, Hye Minko
dc.contributor.authorOh, Young Hoonko
dc.contributor.authorLee, Seung Hwanko
dc.contributor.authorSong, Bong Keunko
dc.contributor.authorJegal, Jong Geonko
dc.contributor.authorLee, Sang Yupko
dc.date.accessioned2016-04-22T07:59:14Z-
dc.date.available2016-04-22T07:59:14Z-
dc.date.created2015-12-31-
dc.date.created2015-12-31-
dc.date.issued2015-11-06-
dc.identifier.citation2015 International Meeting of the Federation of Korean Microbiological Societies-
dc.identifier.urihttp://hdl.handle.net/10203/205789-
dc.description.abstract5-Aminovalerate (5AVA) is an important C5 platform chemical. Escherichia coli was metabolically engineered for the production of 5-aminovalerate (5AVA) and glutarate. When the recombinant E. coli WL3110 strain expressing the Pseudomonas putidadavAB genes encoding delta-aminovaleramidase and lysine 2-monooxygenase, respectively, were cultured and produced 3.6 g/L of 5AVA by converting 7 g/L of l-lysine. When the davAB genes were introduced into recombinant E. coli strain XQ56 allowing enhanced l-lysine synthesis, 0.27 and 0.5 g/L of 5AVA were produced directly from glucose by batch and fed-batch cultures, respectively. Further conversion of 5AVA into glutarate demonstrated by expression of the P. putida gabTD genes encoding 5AVA aminotransferase and glutarate semialdehyde dehydrogenase. When recombinant E. coli WL3110 strain expressing the davAB and gabTD genes was cultured in a medium containing 10 g/L l-lysine and 10 g/L α-ketoglutarate, 1.7 g/L of glutarate was produced.-
dc.languageEnglish-
dc.publisher한국미생물학회연합-
dc.titleAminovalerate and Glutarate Production Pathway Introduced into Escherichia coli-
dc.typeConference-
dc.type.rimsCONF-
dc.citation.publicationname2015 International Meeting of the Federation of Korean Microbiological Societies-
dc.identifier.conferencecountryKO-
dc.identifier.conferencelocation킨텍스 제1전시장 3층-
dc.contributor.localauthorLee, Sang Yup-
dc.contributor.nonIdAuthorKim, Eun Young-
dc.contributor.nonIdAuthorNoh, Won-
dc.contributor.nonIdAuthorPark, Hye Min-
dc.contributor.nonIdAuthorOh, Young Hoon-
dc.contributor.nonIdAuthorSong, Bong Keun-
dc.contributor.nonIdAuthorJegal, Jong Geon-
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CBE-Conference Papers(학술회의논문)
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