Reduction of Adipose Tissue Mass by the Angiogenesis Inhibitor ALS-L1023 from Melissa officinalis

Cited 13 time in webofscience Cited 0 time in scopus
  • Hit : 292
  • Download : 826
It has been suggested that angiogenesis modulates adipogenesis and obesity. This study was undertaken to determine whether ALS-L1023 (ALS) prepared by a two-step organic solvent fractionation from Melissa leaves, which exhibits antiangiogenic activity, can regulate adipose tissue growth. The effects of ALS on angiogenesis and extracellular matrix remodeling were measured using in vitro assays. The effects of ALS on adipose tissue growth were investigated in high fat diet-induced obese mice. ALS inhibited VEGF-and bFGF-induced endothelial cell proliferation and suppressed matrix metalloproteinase (MMP) activity in vitro. Compared to obese control mice, administration of ALS to obese mice reduced body weight gain, adipose tissue mass and adipocyte size without affecting appetite. ALS treatment decreased blood vessel density and MMP activity in adipose tissues. ALS reduced the mRNA levels of angiogenic factors (VEGF-A and FGF-2) and MMPs (MMP-2 and MMP-9), whereas ALS increased the mRNA levels of angiogenic inhibitors (TSP-1, TIMP-1, and TIMP-2) in adipose tissues. The protein levels of VEGF, MMP-2 and MMP-9 were also decreased by ALS in adipose tissue. Metabolic changes in plasma lipids, liver triglycerides, and hepatic expression of fatty acid oxidation genes occurred during ALS-induced weight loss. These results suggest that ALS, which has antiangiogenic and MMP inhibitory activities, reduces adipose tissue mass in nutritionally obese mice, demonstrating that adipose tissue growth can be regulated by angiogenesis inhibitors.
Publisher
PUBLIC LIBRARY SCIENCE
Issue Date
2015-11
Language
English
Article Type
Article
Keywords

MATRIX-METALLOPROTEINASE INHIBITION; ENDOTHELIAL GROWTH-FACTOR; COMPOSITION OB-X; ADIPOCYTE DIFFERENTIATION; INSULIN-RESISTANCE; GELATINASE-A; OBESE MICE; PPAR-ALPHA; MORUS-ALBA; DIET

Citation

PLOS ONE, v.10, no.11

ISSN
1932-6203
DOI
10.1371/journal.pone.0141612
URI
http://hdl.handle.net/10203/205530
Appears in Collection
BS-Journal Papers(저널논문)
Files in This Item
000365853900013.pdf(4.49 MB)Download
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 13 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0