DC Field | Value | Language |
---|---|---|
dc.contributor.author | Bae, Jin Ho | ko |
dc.contributor.author | Jo, Seong-Min | ko |
dc.contributor.author | Kim, Hak-Sung | ko |
dc.date.accessioned | 2016-04-20T06:50:46Z | - |
dc.date.available | 2016-04-20T06:50:46Z | - |
dc.date.created | 2015-10-06 | - |
dc.date.created | 2015-10-06 | - |
dc.date.created | 2015-10-06 | - |
dc.date.issued | 2015-12 | - |
dc.identifier.citation | BIOSENSORS & BIOELECTRONICS, v.74, pp.849 - 855 | - |
dc.identifier.issn | 0956-5663 | - |
dc.identifier.uri | http://hdl.handle.net/10203/205524 | - |
dc.description.abstract | Detection of exon 19 deletion mutation of EGFR, one of the most frequently occurring mutations in lung cancer, provides the crucial information for diagnosis and treatment guideline in non-small-cell lung cancer (NSCLC). Here, we demonstrate a simple and efficient method to detect various exon 19 deletion mutations of EGFR using a single probe set comprising of an oligo-quencher (oligo-Q) and a molecular beacon (MB). While the MB hybridizes to both the wild and mutant target DNA, the oligo-Q only binds to the wild target DNA, leading to a fluorescent signal in case of deletion mutation. This enables the comprehensive detection of the diverse exon 19 deletion mutations using a single probe set. We demonstrated the utility and efficiency of the approach by detecting the frequent exon 19 deletion mutations of EGFR through a real-time PCR and in situ fluorescence imaging. Our approach enabled the detection of genomic DNA as low as 0.02 ng, showing a detection limit of 2% in a heterogeneous DNA mixture, and could be used for detecting mutations in a single cell level. The present MB and oligo-Q dual probe system can be used for diagnosis and treatment guideline in NSCLC. | - |
dc.language | English | - |
dc.publisher | ELSEVIER ADVANCED TECHNOLOGY | - |
dc.title | Comprehensive detection of diverse exon 19 deletion mutations of EGFR in lung Cancer by a single probe set | - |
dc.type | Article | - |
dc.identifier.wosid | 000360772800115 | - |
dc.identifier.scopusid | 2-s2.0-84938251226 | - |
dc.type.rims | ART | - |
dc.citation.volume | 74 | - |
dc.citation.beginningpage | 849 | - |
dc.citation.endingpage | 855 | - |
dc.citation.publicationname | BIOSENSORS & BIOELECTRONICS | - |
dc.identifier.doi | 10.1016/j.bios.2015.07.043 | - |
dc.contributor.localauthor | Kim, Hak-Sung | - |
dc.description.isOpenAccess | N | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Exon 19 deletion mutations | - |
dc.subject.keywordAuthor | Oligo-quencher | - |
dc.subject.keywordAuthor | Molecular beacon | - |
dc.subject.keywordAuthor | Lung cancer | - |
dc.subject.keywordAuthor | EGER | - |
dc.subject.keywordPlus | GROWTH-FACTOR-RECEPTOR | - |
dc.subject.keywordPlus | ACID HYBRIDIZATION PROBES | - |
dc.subject.keywordPlus | MOLECULAR BEACONS | - |
dc.subject.keywordPlus | NUCLEIC-ACID | - |
dc.subject.keywordPlus | RAPID DETECTION | - |
dc.subject.keywordPlus | GEFITINIB | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | BIOMARKER | - |
dc.subject.keywordPlus | SURVIVAL | - |
dc.subject.keywordPlus | ASSAY | - |
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