Proteome-based identification of fusion partner for high-level extracellular production of recombinant proteins in Escherichia coli

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dc.contributor.authorQian, ZGko
dc.contributor.authorXia, XXko
dc.contributor.authorChoi, JHko
dc.contributor.authorLee, SangYupko
dc.date.accessioned2010-11-24T08:09:40Z-
dc.date.available2010-11-24T08:09:40Z-
dc.date.created2012-02-06-
dc.date.created2012-02-06-
dc.date.issued2008-10-
dc.identifier.citationBIOTECHNOLOGY AND BIOENGINEERING, v.101, no.3, pp.587 - 601-
dc.identifier.issn0006-3592-
dc.identifier.urihttp://hdl.handle.net/10203/20336-
dc.description.abstractExtracellular production of recombinant proteins in Escherichia coli has several advantages over cytoplasmic or periplasmic production. However, nonpathogenic laboratory strains of E. coli generally excrete only trace amounts of proteins into the Culture medium Under normal growth conditions. Here we report a systematic proteome-based approach for developing a system for high-level extracellular production of recombinant proteins in E. coli. First, we analyzed the extracellular proteome of an E. coli B strain, BL21(DE3), to identify naturally excreted proteins, assuming that these proteins may serve as potential fusion partners for the production of recombinant proteins in the medium. Next, overexpression and excretion Studies were performed for the 20 selected fusion partners with molecular weights below 40 kDa. Twelve of them were found to allow fused proteins to excrete into the medium at considerable levels. The most efficient excreting fusion partner, OsmY, was used as a carrier protein to excrete heterologous proteins into the medium. E. coli alkaline phosphatase, Bacillus subtilis alpha-amylase, and human leptin used as model proteins could all be excreted into the medium at concentrations ranging from 5 to 64 mg/L during the flask cultivation. When only the signal peptide or the mature part of OsmY was used as a fusion partner, no such excretion was observed; this confirmed that these proteins were truly excreted rather than released by outer membrane leakage. The recombinant protein of interest could be recovered by cleaving off the fusion partner by enterokinase as demonstrated for alkaline phosphatase as an example. High cell density cultivation allowed production of these proteins to the levels of 250-700 mg/L in the culture medium, suggesting the good potential of this approach for the excretory production of recombinant proteins.-
dc.description.sponsorshipThis work was a part of the new fusion research initiative supported by the KAIST Institute for the BioCentury.en
dc.languageEnglish-
dc.language.isoen_USen
dc.publisherWILEY-BLACKWELL-
dc.subjectCELL-DENSITY CULTIVATION-
dc.subjectCULTURE-MEDIUM-
dc.subjectHUMAN LEPTIN-
dc.subjectSECRETION-
dc.subjectPURIFICATION-
dc.subjectEXPRESSION-
dc.subjectEFFICIENT-
dc.subjectPATHWAY-
dc.subjectOVEREXPRESSION-
dc.subjectLIPOPROTEIN-
dc.titleProteome-based identification of fusion partner for high-level extracellular production of recombinant proteins in Escherichia coli-
dc.typeArticle-
dc.identifier.wosid000259358700016-
dc.identifier.scopusid2-s2.0-53549088613-
dc.type.rimsART-
dc.citation.volume101-
dc.citation.issue3-
dc.citation.beginningpage587-
dc.citation.endingpage601-
dc.citation.publicationnameBIOTECHNOLOGY AND BIOENGINEERING-
dc.identifier.doi10.1002/bit.21898-
dc.embargo.liftdate9999-12-31-
dc.embargo.terms9999-12-31-
dc.contributor.localauthorLee, SangYup-
dc.contributor.nonIdAuthorQian, ZG-
dc.contributor.nonIdAuthorXia, XX-
dc.contributor.nonIdAuthorChoi, JH-
dc.type.journalArticleArticle-
dc.subject.keywordAuthorextracellular proteome-
dc.subject.keywordAuthorEscherichia coli-
dc.subject.keywordAuthorrecombinant protein-
dc.subject.keywordAuthorOsmY-
dc.subject.keywordAuthorhigh cell density cultivation-
dc.subject.keywordPlusCELL-DENSITY CULTIVATION-
dc.subject.keywordPlusCULTURE-MEDIUM-
dc.subject.keywordPlusHUMAN LEPTIN-
dc.subject.keywordPlusSECRETION-
dc.subject.keywordPlusPURIFICATION-
dc.subject.keywordPlusEXPRESSION-
dc.subject.keywordPlusEFFICIENT-
dc.subject.keywordPlusPATHWAY-
dc.subject.keywordPlusOVEREXPRESSION-
dc.subject.keywordPlusLIPOPROTEIN-
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