A complex system such as defense systems is in a form of System of Systems (SoS) in which each component is a system being independent of other component systems. Dynamical behavior of SoS is represented by a composition of behaviors of component systems. Thus, a M&S tool/environment would not be efficient for development of heterogeneous models nor for simulation in a centralized environment. Moreover, such an environment restricts reusability and composability. This paper presents an interoperation method for M&S of defense systems as SoS. The approach first develops component system models using tools, each specialized to M&S of each component system. It then interoperates such simulations together to simulate a whole system as SoS. HLA/RTI is employed for such interoperation, which is a DoD/IEEE standard to support interoperation. We will introduce a case study for interoperable simulation of defense systems, which consists of a wargame simulator and a communication simulator.