DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Su-Hyung | ko |
dc.contributor.author | Yang, SH | ko |
dc.contributor.author | Lee, CG | ko |
dc.contributor.author | Youn, JW | ko |
dc.contributor.author | Chang, J | ko |
dc.contributor.author | Sung, YC | ko |
dc.date.accessioned | 2015-11-20T10:28:51Z | - |
dc.date.available | 2015-11-20T10:28:51Z | - |
dc.date.created | 2014-10-02 | - |
dc.date.created | 2014-10-02 | - |
dc.date.issued | 2003-11 | - |
dc.identifier.citation | VACCINE, v.21, no.31, pp.4555 - 4564 | - |
dc.identifier.issn | 0264-410X | - |
dc.identifier.uri | http://hdl.handle.net/10203/201434 | - |
dc.description.abstract | Hepatitis C virus (HCV) is an important causative agent of liver disease, but currently there is no available prophylactic vaccine against HCV infection. Here, we investigated the HCV E2- and core-specific T-cell responses induced by DNA (D) and/or recombinant adenovirus (A) vaccines. In single (D versus A) or double immunizations (D-D versus A-A), the recombinant adenovirus vaccines induced higher levels of IFN-gamma secreting T-cell response and cytotoxic T lymphocytes (CTL) response than the DNA vaccines. However, a heterologous (D-A) regimen elicited the highest level of T helper 1 (Th1) CD4(+) T-cell responses. Furthermore, three E2-specific CTL epitopes were mapped using a peptide pool spanning the E2 protein sequence (a.a. 384-713) in BALB/c mice, and one of these (E2 405-414: SGPSQKIQLV) was shown to be immunodominant. Interestingly, no significant differences were found in the repertoire of E2-specific T-cell responses or in the immunodominance hierarchy of the three epitopes induced by D-D, D-A, A-A, and A-D, indicating that the breadth and hierarchy of T-cell responses is independent of these different vaccination regimens. In conclusion, the heterologous DNA prime-recombinant adenovirus boost regimen described offers an efficient promising strategy for the development of an effective T-cell-based HCV vaccine. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.subject | RECOMBINANT ADENOVIRUSES | - |
dc.subject | LYMPHOCYTE RESPONSES | - |
dc.subject | PLASMID DNA | - |
dc.subject | INFECTION | - |
dc.subject | PROTEINS | - |
dc.subject | VACCINE | - |
dc.subject | IMMUNIZATION | - |
dc.subject | MECHANISM | - |
dc.subject | MICE | - |
dc.title | Efficient induction of T helper 1 CD4(+) T-cell responses to hepatitis C virus core and E2 by a DNA prime-adenovirus boost | - |
dc.type | Article | - |
dc.identifier.wosid | 000186830900010 | - |
dc.identifier.scopusid | 2-s2.0-0142231018 | - |
dc.type.rims | ART | - |
dc.citation.volume | 21 | - |
dc.citation.issue | 31 | - |
dc.citation.beginningpage | 4555 | - |
dc.citation.endingpage | 4564 | - |
dc.citation.publicationname | VACCINE | - |
dc.identifier.doi | 10.1016/S0264-410X(03)00499-7 | - |
dc.contributor.localauthor | Park, Su-Hyung | - |
dc.contributor.nonIdAuthor | Yang, SH | - |
dc.contributor.nonIdAuthor | Lee, CG | - |
dc.contributor.nonIdAuthor | Youn, JW | - |
dc.contributor.nonIdAuthor | Chang, J | - |
dc.contributor.nonIdAuthor | Sung, YC | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | hepatitis C virus | - |
dc.subject.keywordAuthor | DNA prime-recombinant adenovirus boost | - |
dc.subject.keywordAuthor | Th1CD4(+) T-cell response | - |
dc.subject.keywordPlus | RECOMBINANT ADENOVIRUSES | - |
dc.subject.keywordPlus | LYMPHOCYTE RESPONSES | - |
dc.subject.keywordPlus | PLASMID DNA | - |
dc.subject.keywordPlus | INFECTION | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | VACCINE | - |
dc.subject.keywordPlus | IMMUNIZATION | - |
dc.subject.keywordPlus | MECHANISM | - |
dc.subject.keywordPlus | MICE | - |
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