Structural characterization of a human-type corrinoid adenosyltransferase confirms that coenzyme B-12 is synthesized through a four-coordinate intermediate

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ATP:cob(I)alamin adenosyltransferases (ACAs) catalyze, the transfer of the 5'-deoxyadenosyl moiety from ATP to the upper axial ligand position of cobalamin in the synthesis of coenzyme B-12. For the ACA-catalyzed reaction to proceed, cob(II)alamin must be reduced to cob(I)alamin in the enzyme active site. This reduction is facilitated through the generation of a four-coordinate cob(II)alamin intermediate on the enzyme. We have determined the high-resolution crystal structure of a human-type ACA from Lactobacillus reuteri with a four-coordinate cob(II)alamin bound in the enzyme active site and with the product, adenosylcobalamin, partially occupied in the active site. The assembled structures represent snapshots of the steps in the ACA-catalyzed formation of the cobalt-carbon bond of coenzyme B-12. The structures define the corrinoid binding site and provide visual evidence for a base-off, four-coordinate cob(II)alamin intermediate. The complete structural description of ACA-mediated catalysis reveals the molecular features of four-coordinate cob(II)alamin stabilization and provides additional insights into the molecular basis for dysfunction in human patients suffering from methylmalonic aciduria.
Publisher
AMER CHEMICAL SOC
Issue Date
2008-05
Language
English
Article Type
Article
Keywords

METHYLMALONYL-COA MUTASE; X-RAY-STRUCTURE; SALMONELLA-ENTERICA; ATP-COB(I)ALAMIN ADENOSYLTRANSFERASE; COBALAMIN ADENOSYLTRANSFERASE; ELECTRONIC-PROPERTIES; METHIONINE SYNTHASE; CO2+ COBALAMIN; ATP-CO(I)RRINOID ADENOSYLTRANSFERASE; INITIAL-CHARACTERIZATION

Citation

BIOCHEMISTRY, v.47, no.21, pp.5755 - 5766

ISSN
0006-2960
DOI
10.1021/bi800132d
URI
http://hdl.handle.net/10203/201427
Appears in Collection
CH-Journal Papers(저널논문)
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