Ubiquitous Detection of Gram-Positive Bacteria with Bioorthogonal Magnetofluorescent Nanoparticles

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The ability to rapidly diagnose gram-positive pathogenic bacteria would have far reaching biomedical and technological applications. Here we describe the bloorthogonal modification of small molecule antibiotics (vancomycin and daptomycin), which bind to the cell wall of gram-positive bacteria. The bound antibiotics conjugates can be reacted orthogonally with tetrazine-modified nanoparticles, via an almost Instantaneous cycloaddition, which subsequently renders the bacteria detectable by optical or magnetic sensing. We show that this approach is specific, selective, fast and biocompatible. Furthermore, it can be adapted to the detection of intracellular pathogens. Importantly, this strategy enables detection of entire classes of bacteria, a feat that is difficult to achieve using current antibody approaches. Compared to covalent nanoparticle conjugates, our bioorthogonal method demonstrated 1-2 orders of magnitude greater sensitivity. This bioorthogonal labeling method could ultimately be applied to a variety of other small molecules with specificity for infectious pathogens, enabling their detection and diagnosis.
Publisher
AMER CHEMICAL SOC
Issue Date
2011-11
Language
English
Article Type
Article
Keywords

SELF-ASSEMBLED MONOLAYERS; MAGNETIC NANOPARTICLES; CANCER-CELLS; VANCOMYCIN; DAPTOMYCIN; INFECTIONS; BINDING; ANTIBIOTICS; ENTEROCOCCI

Citation

ACS NANO, v.5, no.11, pp.8834 - 8841

ISSN
1936-0851
DOI
10.1021/nn2029692
URI
http://hdl.handle.net/10203/201392
Appears in Collection
NT-Journal Papers(저널논문)
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