Incorporating metabolic flux ratios into constraint-based flux analysis by using artificial metabolites and converging ratio determinants

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One of the well-established approaches for the quantitative characterization of large-scale underdetermined metabolic network is constraint-based flux analysis, which quantifies intracellular metabolic fluxes to characterize the metabolic status. The system is typically underdetermined, and thus usually is solved by linear programming with the measured external fluxes as constraints. Thus, the intracellular flux distribution calculated may not represent the true values. C-13-constrained flux analysis allows more accurate determination of internal fluxes, but is currently limited to relatively small metabolic networks due to the requirement of complicated mathematical formulation and limited parameters available. Here, we report a strategy of employing such partial information obtained from the C-13-labeling experiments as additional constraints during the constraint-based flux analysis. A new methodology employing artificial metabolites and converging ratio determinants (CRDs) was developed for improving constraint-based flux analysis. The CRDs were determined based on the metabolic flux ratios obtained from C-13-labeling experiments, and were incorporated into the mass balance equations for the artificial metabolites. These new mass balance equations were used as additional constraints during the constraint-based flux analysis with genome-scale E. coli metabolic model, which allowed more accurate determination of intracellular metabolic fluxes. (c) 2007 Elsevier B.V. All rights reserved.
Publisher
ELSEVIER SCIENCE BV
Issue Date
2007-05
Language
English
Article Type
Article
Keywords

MASS-SPECTROMETRY; ESCHERICHIA-COLI; PATHWAY ANALYSIS; NETWORKS; MODELS; METAFLUXNET; SYSTEMS; DESIGN

Citation

JOURNAL OF BIOTECHNOLOGY, v.129, pp.696 - 705

ISSN
0168-1656
DOI
10.1016/j.jbiotec.2007.02.026
URI
http://hdl.handle.net/10203/20126
Appears in Collection
CBE-Journal Papers(저널논문)
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