Geometric effect of the hydrogel grid structure on in vitro formation of homogeneous MIN6 cell clusters

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A microstructure-based hydrogel was employed to study the relationship between spatial specificity and cellular behavior, including cell fate, proliferation, morphology, and insulin secretion in pancreatic beta-cells. To effectively form homogeneous cell clusters in vitro, we made cell-containing hydrogel membrane constructs with an adapted grid structure based on a hexagonal micropattern. Homogeneous cell clusters (average diameter: 83.6 +/- 14.2 mu m) of pancreatic insulinoma (MIN6) cells were spontaneously generated in the floating hydrogel membrane constructs, including a hexagonal grid structure (size of cavity: 100 mu m, interval between cavities: 30 mu m). Interestingly, 3D clustering of MIN6 cells mimicking the structure of pancreatic islets was coalesced into a merged aggregate attaching to each hexagonal cavity of the hydrogel grid structure. The fate and insulin secretion of homogeneous cell clusters in the hydrogel grid structure were also assessed. The results of these designable hydrogel-cell membrane constructs suggest that facultative in vitro beta-cell proliferation and maintenance can be applied to biofunctional assessments.
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2014-06
Language
English
Article Type
Article
Keywords

INSULIN SECRETORY RESPONSES; PANCREATIC-ISLET SPHEROIDS; CULTURE PLATFORM; BETA-CELLS; 3D; MICROENVIRONMENTS; ORGANIZATION

Citation

LAB ON A CHIP, v.14, no.13, pp.2183 - 2190

ISSN
1473-0197
DOI
10.1039/c3lc51421h
URI
http://hdl.handle.net/10203/201243
Appears in Collection
MSE-Journal Papers(저널논문)BiS-Journal Papers(저널논문)
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