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College of Life Science and Bioengineering(생명과학기술대학)Dept. of Biological Sciences(생명과학과)BS-Journal Papers(저널논문)

A selective fluorescent probe for cysteine and its imaging in live cells

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dc.contributor.authorKim, Youngsamko
dc.contributor.authorChoi, Minsukko
dc.contributor.authorSeo, Seokjunko
dc.contributor.authorManjare, Sudesh T.ko
dc.contributor.authorJon, Sangyongko
dc.contributor.authorChurchill, David Gko
dc.date.accessioned2015-11-20T09:51:06Z-
dc.date.available2015-11-20T09:51:06Z-
dc.date.created2014-11-25-
dc.date.created2014-11-25-
dc.date.issued2014-11-
dc.identifier.citationRSC ADVANCES, v.4, no.109, pp.64183 - 64186-
dc.identifier.issn2046-2069-
dc.identifier.urihttp://hdl.handle.net/10203/201124-
dc.description.abstractA probe for the detection of cysteine over homocysteine based on 2-(2'-hydroxyphenyl) benzothiazole (HBT) was prepared and used in confocal microscopy experiments. The probe was designed to block excited state intramolecular proton transfer (ESIPT). When bromopropionyl group protection is removed, HBT is recovered via nucleophilic substitution and intramolecular cyclization. The probe was found to have a detection limit of 2.8 mu M and exhibits a similar to 20-fold increase. The probe showed cell membrane permeability and efficacy in living Hep3B cells.-
dc.languageEnglish-
dc.publisherROYAL SOC CHEMISTRY-
dc.subjectASSISTED ELECTROSTATIC ATTRACTION-
dc.subjectPROTEIN FUNCTION-
dc.subjectLIVING CELLS-
dc.subjectHOMOCYSTEINE-
dc.subjectGLUTATHIONE-
dc.subjectMICROSCOPY-
dc.subjectCONJUGATE-
dc.titleA selective fluorescent probe for cysteine and its imaging in live cells-
dc.typeArticle-
dc.identifier.wosid000345702900075-
dc.identifier.scopusid2-s2.0-84914172570-
dc.type.rimsART-
dc.citation.volume4-
dc.citation.issue109-
dc.citation.beginningpage64183-
dc.citation.endingpage64186-
dc.citation.publicationnameRSC ADVANCES-
dc.identifier.doi10.1039/C4RA12981D-
dc.contributor.localauthorJon, Sangyong-
dc.contributor.localauthorChurchill, David G-
dc.contributor.nonIdAuthorSeo, Seokjun-
dc.contributor.nonIdAuthorManjare, Sudesh T.-
dc.type.journalArticleArticle-
dc.subject.keywordPlusASSISTED ELECTROSTATIC ATTRACTION-
dc.subject.keywordPlusPROTEIN FUNCTION-
dc.subject.keywordPlusLIVING CELLS-
dc.subject.keywordPlusHOMOCYSTEINE-
dc.subject.keywordPlusGLUTATHIONE-
dc.subject.keywordPlusMICROSCOPY-
dc.subject.keywordPlusCONJUGATE-
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BS-Journal Papers(저널논문)CH-Journal Papers(저널논문)
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