DC Field | Value | Language |
---|---|---|
dc.contributor.author | Park, Seonhwa | ko |
dc.contributor.author | Kim, Jihye | ko |
dc.contributor.author | Ock, Hwiseok | ko |
dc.contributor.author | Dutta, Gorachand | ko |
dc.contributor.author | Seo, Jeongwook | ko |
dc.contributor.author | Shin, Eui-Cheol | ko |
dc.contributor.author | Yang, Haesik | ko |
dc.date.accessioned | 2015-11-20T07:38:31Z | - |
dc.date.available | 2015-11-20T07:38:31Z | - |
dc.date.created | 2015-08-25 | - |
dc.date.created | 2015-08-25 | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | ANALYST, v.140, no.16, pp.5481 - 5487 | - |
dc.identifier.issn | 0003-2654 | - |
dc.identifier.uri | http://hdl.handle.net/10203/200794 | - |
dc.description.abstract | Washing processes cannot fully remove interfering species that remain on biosensing surfaces when a sample solution contains a high concentration of interfering species. This study reports an immunosensing scheme employing electroreduction-based electrochemical-chemical (EC) redox cycling that allows sensitive detection of vaccinia virus (VV) in a solution containing a high concentration of L-ascorbic acid (AA). To obtain high signal amplification, an enzymatic reaction by beta-D-galactosidase (Gal) is combined with electroreduction-based EC redox cycling by an oxidant. Among the four possible oxidants (KIO3, NaClO, Ag2O, and H2O2), KIO3 shows the highest signal-to-background ratio and is chosen. During an incubation period of 10 min, Gal converts beta-D-galactopyranoside into p-aminophenol (AP), which is oxidized to p-quinone imine (QI) by KIO3. When -0.05 V vs. Ag/AgCl is applied to an immunosensing electrode, QI is reduced to AP, and the regenerated AP is then reoxidized by KIO3. The electroreduction-based EC redox cycling is induced. An indium-tin oxide electrode modified with reduced graphene oxide and an applied potential of -0.05 V are used to achieve low and reproducible background currents, slow O-2 reduction, and fast electroreduction of QI. KIO3 favorably converts AA into noninterfering species during the incubation period. The detection limit for VV in commercial 50% mandarin juice (AA concentration = 0.7 mM) is 4 x 10(3) plaque-forming unit (PFU) per mL. The new EC redox cycling scheme is promising for sensitive detection of proteins, viruses, and bacteria in solutions containing high concentrations of AA. | - |
dc.language | English | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | TIN OXIDE ELECTRODES | - |
dc.subject | CARBON NANOTUBE | - |
dc.subject | INFLUENZA-VIRUS | - |
dc.subject | URIC-ACID | - |
dc.subject | BIOSENSOR | - |
dc.subject | DNA | - |
dc.subject | INTERFERENCE | - |
dc.subject | IMMUNOSENSOR | - |
dc.subject | AMPLIFICATION | - |
dc.subject | LABEL | - |
dc.title | Sensitive electrochemical detection of vaccinia virus in a solution containing a high concentration of L-ascorbic acid | - |
dc.type | Article | - |
dc.identifier.wosid | 000358521400010 | - |
dc.identifier.scopusid | 2-s2.0-84938410561 | - |
dc.type.rims | ART | - |
dc.citation.volume | 140 | - |
dc.citation.issue | 16 | - |
dc.citation.beginningpage | 5481 | - |
dc.citation.endingpage | 5487 | - |
dc.citation.publicationname | ANALYST | - |
dc.identifier.doi | 10.1039/c5an01086a | - |
dc.contributor.localauthor | Shin, Eui-Cheol | - |
dc.contributor.nonIdAuthor | Park, Seonhwa | - |
dc.contributor.nonIdAuthor | Ock, Hwiseok | - |
dc.contributor.nonIdAuthor | Dutta, Gorachand | - |
dc.contributor.nonIdAuthor | Seo, Jeongwook | - |
dc.contributor.nonIdAuthor | Yang, Haesik | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | TIN OXIDE ELECTRODES | - |
dc.subject.keywordPlus | CARBON NANOTUBE | - |
dc.subject.keywordPlus | INFLUENZA-VIRUS | - |
dc.subject.keywordPlus | BIOSENSORS | - |
dc.subject.keywordPlus | DNA | - |
dc.subject.keywordPlus | IMMUNOSENSOR | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | LABEL | - |
dc.subject.keywordPlus | FILM | - |
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