DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, Choong | ko |
dc.contributor.author | Kasuya, Junichi | ko |
dc.contributor.author | Jeon, Jessie Sungyun | ko |
dc.contributor.author | Chung, Seok | ko |
dc.contributor.author | Kamm, Roger D. | ko |
dc.date.accessioned | 2015-11-20T07:38:05Z | - |
dc.date.available | 2015-11-20T07:38:05Z | - |
dc.date.created | 2015-08-04 | - |
dc.date.created | 2015-08-04 | - |
dc.date.created | 2015-08-04 | - |
dc.date.issued | 2015-01 | - |
dc.identifier.citation | LAB ON A CHIP, v.15, no.1, pp.301 - 310 | - |
dc.identifier.issn | 1473-0197 | - |
dc.identifier.uri | http://hdl.handle.net/10203/200790 | - |
dc.description.abstract | "Anti-angiogenic therapy, which suppresses tumor growth by disrupting oxygen and nutrient supply from blood to the tumor, is now widely accepted as a treatment for cancer. To investigate the mechanisms of action of these anti-angiogenesis drugs, new three dimensional (3D) cell culture-based drug screening models are increasingly employed. However, there is no in vitro high-throughput screening (HTS) angiogenesis assay that can provide uniform culture conditions for the quantitative assessment of physiological responses to chemoattractant reagents under various concentrations of anti-angiogenesis drugs. Here we describe a method for screening and quantifying the vascular endothelial growth factor (VEGF)-induced chemotactic response on human umbilical vein endothelial cells (HUVECs) cultured with different concentrations of bortezomib, a selective 26S proteasome inhibitor. With this quantitative microfluidic angiogenesis screen (QMAS), we demonstrate that bortezomib-induced endothelial cell death is preceded by a series of morphological changes that develop over several days. We also explore the mechanisms by which bortezomib can inhibit angiogenesis." | - |
dc.language | English | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.subject | IN-VITRO | - |
dc.subject | CELL-MIGRATION | - |
dc.subject | ANTICANCER DRUGS | - |
dc.subject | TUMOR SPHEROIDS | - |
dc.subject | PLATFORM | - |
dc.subject | CANCER | - |
dc.subject | BORTEZOMIB | - |
dc.subject | CULTURE | - |
dc.subject | MORPHOGENESIS | - |
dc.subject | THERAPIES | - |
dc.title | A quantitative microfluidic angiogenesis screen for studying anti-angiogenic therapeutic drugs | - |
dc.type | Article | - |
dc.identifier.wosid | 000346478100035 | - |
dc.identifier.scopusid | 2-s2.0-84915793469 | - |
dc.type.rims | ART | - |
dc.citation.volume | 15 | - |
dc.citation.issue | 1 | - |
dc.citation.beginningpage | 301 | - |
dc.citation.endingpage | 310 | - |
dc.citation.publicationname | LAB ON A CHIP | - |
dc.identifier.doi | 10.1039/c4lc00866a | - |
dc.contributor.localauthor | Jeon, Jessie Sungyun | - |
dc.contributor.nonIdAuthor | Kim, Choong | - |
dc.contributor.nonIdAuthor | Kasuya, Junichi | - |
dc.contributor.nonIdAuthor | Chung, Seok | - |
dc.contributor.nonIdAuthor | Kamm, Roger D. | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | CELL-MIGRATION | - |
dc.subject.keywordPlus | ANTICANCER DRUGS | - |
dc.subject.keywordPlus | TUMOR SPHEROIDS | - |
dc.subject.keywordPlus | PLATFORM | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | BORTEZOMIB | - |
dc.subject.keywordPlus | CULTURE | - |
dc.subject.keywordPlus | MORPHOGENESIS | - |
dc.subject.keywordPlus | THERAPIES | - |
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