A quantitative microfluidic angiogenesis screen for studying anti-angiogenic therapeutic drugs

Cited 72 time in webofscience Cited 0 time in scopus
  • Hit : 303
  • Download : 0
"Anti-angiogenic therapy, which suppresses tumor growth by disrupting oxygen and nutrient supply from blood to the tumor, is now widely accepted as a treatment for cancer. To investigate the mechanisms of action of these anti-angiogenesis drugs, new three dimensional (3D) cell culture-based drug screening models are increasingly employed. However, there is no in vitro high-throughput screening (HTS) angiogenesis assay that can provide uniform culture conditions for the quantitative assessment of physiological responses to chemoattractant reagents under various concentrations of anti-angiogenesis drugs. Here we describe a method for screening and quantifying the vascular endothelial growth factor (VEGF)-induced chemotactic response on human umbilical vein endothelial cells (HUVECs) cultured with different concentrations of bortezomib, a selective 26S proteasome inhibitor. With this quantitative microfluidic angiogenesis screen (QMAS), we demonstrate that bortezomib-induced endothelial cell death is preceded by a series of morphological changes that develop over several days. We also explore the mechanisms by which bortezomib can inhibit angiogenesis."
Publisher
ROYAL SOC CHEMISTRY
Issue Date
2015-01
Language
English
Article Type
Article
Keywords

IN-VITRO; CELL-MIGRATION; ANTICANCER DRUGS; TUMOR SPHEROIDS; PLATFORM; CANCER; BORTEZOMIB; CULTURE; MORPHOGENESIS; THERAPIES

Citation

LAB ON A CHIP, v.15, no.1, pp.301 - 310

ISSN
1473-0197
DOI
10.1039/c4lc00866a
URI
http://hdl.handle.net/10203/200790
Appears in Collection
ME-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 72 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0