DC Field | Value | Language |
---|---|---|
dc.contributor.author | Jang, Ju Hye | ko |
dc.contributor.author | Kim, Yu Jin | ko |
dc.contributor.author | Kim, Hyun | ko |
dc.contributor.author | Kim, Sun-Chang | ko |
dc.contributor.author | Cho, JH | ko |
dc.date.accessioned | 2015-06-29T04:41:22Z | - |
dc.date.available | 2015-06-29T04:41:22Z | - |
dc.date.created | 2015-06-23 | - |
dc.date.created | 2015-06-23 | - |
dc.date.issued | 2015-07 | - |
dc.identifier.citation | PEPTIDES, v.69, pp.144 - 149 | - |
dc.identifier.issn | 0196-9781 | - |
dc.identifier.uri | http://hdl.handle.net/10203/199450 | - |
dc.description.abstract | Buforin IIb, a novel cell-penetrating anticancer peptide derived from histone H2A, has been reported to induce mitochondria-dependent apoptosis in tumor cells. However, increasing evidence suggests that endoplasmic reticulum and mitochondria cooperate to signal cell death. In this study, we investigated the mechanism of buforin IIb-induced apoptosis in human cervical carcinoma HeLa cells by focusing on ER stress-mediated mitochondrial membrane permeabilization. Two-dimensional PAGE coupled with MALDI-TOF and western blot analysis showed that buforin IIb treatment of HeLa cells resulted in upregulation of ER stress proteins. PBA (ER stress inhibitor) and BAPTA/AM (Ca2+ chelator) pretreatment rescued viability of buforin IIb-treated cells through abolishing phosphorylation of SAPK/JNK and p38 MAPK. SP600125 (SAPK/JNK inhibitor) and SB203580 (p38 MAPK inhibitor) attenuated down-regulation of Bcl-xL/Bcl-2, mitochondrial translocation of Bax, and cytochrome c release from mitochondria. Taken together, our data suggest that the ER stress pathway has an important role in the buforin IIb-induced apoptosis in HeLa cells. | - |
dc.language | English | - |
dc.publisher | ELSEVIER SCIENCE INC | - |
dc.subject | UNFOLDED PROTEIN RESPONSE | - |
dc.subject | BCL-2 FAMILY | - |
dc.subject | ER STRESS | - |
dc.subject | ELECTROPHORESIS DATABASE | - |
dc.subject | ANTIMICROBIAL PEPTIDE | - |
dc.subject | POLYACRYLAMIDE-GEL | - |
dc.subject | MITOCHONDRIA | - |
dc.subject | ROLES | - |
dc.subject | IDENTIFICATION | - |
dc.subject | MECHANISM | - |
dc.title | Buforin IIb induces endoplasmic reticulum stress-mediated apoptosis in HeLa cells | - |
dc.type | Article | - |
dc.identifier.wosid | 000355214300020 | - |
dc.identifier.scopusid | 2-s2.0-84930203261 | - |
dc.type.rims | ART | - |
dc.citation.volume | 69 | - |
dc.citation.beginningpage | 144 | - |
dc.citation.endingpage | 149 | - |
dc.citation.publicationname | PEPTIDES | - |
dc.identifier.doi | 10.1016/j.peptides.2015.04.024 | - |
dc.contributor.localauthor | Kim, Sun-Chang | - |
dc.contributor.nonIdAuthor | Jang, Ju Hye | - |
dc.contributor.nonIdAuthor | Kim, Yu Jin | - |
dc.contributor.nonIdAuthor | Kim, Hyun | - |
dc.contributor.nonIdAuthor | Cho, JH | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | Buforin IIb | - |
dc.subject.keywordAuthor | Anticancer peptide | - |
dc.subject.keywordAuthor | Apotosis | - |
dc.subject.keywordAuthor | ER stress | - |
dc.subject.keywordAuthor | Mitochondrial membrane permeabilization | - |
dc.subject.keywordPlus | UNFOLDED PROTEIN RESPONSE | - |
dc.subject.keywordPlus | BCL-2 FAMILY | - |
dc.subject.keywordPlus | ER STRESS | - |
dc.subject.keywordPlus | ELECTROPHORESIS DATABASE | - |
dc.subject.keywordPlus | ANTIMICROBIAL PEPTIDE | - |
dc.subject.keywordPlus | POLYACRYLAMIDE-GEL | - |
dc.subject.keywordPlus | MITOCHONDRIA | - |
dc.subject.keywordPlus | ROLES | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | MECHANISM | - |
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