DC Field | Value | Language |
---|---|---|
dc.contributor.author | Gang, Hyun Ah | ko |
dc.contributor.author | Shin, Ho-Chul | ko |
dc.contributor.author | Kalantzi, Alexandra-Styliani | ko |
dc.contributor.author | Toseland, Christopher P. | ko |
dc.contributor.author | Kim, Hyun-Min | ko |
dc.contributor.author | Gruber, Stephan | ko |
dc.contributor.author | Dal Peraro, Matteo | ko |
dc.contributor.author | Oh, Byung-Ha | ko |
dc.date.accessioned | 2015-06-25T06:27:30Z | - |
dc.date.available | 2015-06-25T06:27:30Z | - |
dc.date.created | 2015-05-06 | - |
dc.date.created | 2015-05-06 | - |
dc.date.created | 2015-05-06 | - |
dc.date.issued | 2015-04 | - |
dc.identifier.citation | NUCLEIC ACIDS RESEARCH, v.43, no.7, pp.3841 - 3856 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.uri | http://hdl.handle.net/10203/199068 | - |
dc.description.abstract | In meiotic DNA recombination, the Hop2-Mnd1 complex promotes Dmc1-mediated single-stranded DNA (ssDNA) invasion into homologous chromosomes to form a synaptic complex by a yet-unclear mechanism. Here, the crystal structure of Hop2-Mnd1 reveals that it forms a curved rod-like structure consisting of three leucine zippers and two kinked junctions. One end of the rod is linked to two juxtaposed winged-helix domains, and the other end is capped by extra alpha-helices to form a helical bundle-like structure. Deletion analysis shows that the helical bundle-like structure is sufficient for interacting with the Dmc1-ssDNA nucleofilament, and molecular modeling suggests that the curved rod could be accommodated into the helical groove of the nucleofilament. Remarkably, the winged-helix domains are juxtaposed at fixed relative orientation, and their binding to DNA is likely to perturb the base pairing according to molecular simulations. These findings allow us to propose a model explaining how Hop2-Mnd1 juxtaposes Dmc1-bound ssDNA with distorted recipient double-stranded DNA and thus facilitates strand invasion. | - |
dc.language | English | - |
dc.publisher | OXFORD UNIV PRESS | - |
dc.subject | DOUBLE-STRAND BREAKS | - |
dc.subject | NUCLEIC-ACID STRUCTURES | - |
dc.subject | PROTEIN COMPLEX | - |
dc.subject | FISSION YEAST | - |
dc.subject | SACCHAROMYCES-CEREVISIAE | - |
dc.subject | RAD51 RECOMBINASE | - |
dc.subject | DNA RECOMBINATION | - |
dc.subject | HOP2 PROTEIN | - |
dc.subject | MEIOSIS | - |
dc.subject | DMC1 | - |
dc.title | Crystal structure of Hop2-Mnd1 and mechanistic insights into its role in meiotic recombination | - |
dc.type | Article | - |
dc.identifier.wosid | 000354722500042 | - |
dc.identifier.scopusid | 2-s2.0-84930506357 | - |
dc.type.rims | ART | - |
dc.citation.volume | 43 | - |
dc.citation.issue | 7 | - |
dc.citation.beginningpage | 3841 | - |
dc.citation.endingpage | 3856 | - |
dc.citation.publicationname | NUCLEIC ACIDS RESEARCH | - |
dc.identifier.doi | 10.1093/nar/gkv172 | - |
dc.embargo.liftdate | 9999-12-31 | - |
dc.embargo.terms | 9999-12-31 | - |
dc.contributor.localauthor | Oh, Byung-Ha | - |
dc.contributor.nonIdAuthor | Kalantzi, Alexandra-Styliani | - |
dc.contributor.nonIdAuthor | Toseland, Christopher P. | - |
dc.contributor.nonIdAuthor | Gruber, Stephan | - |
dc.contributor.nonIdAuthor | Dal Peraro, Matteo | - |
dc.description.isOpenAccess | Y | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordPlus | DOUBLE-STRAND BREAKS | - |
dc.subject.keywordPlus | NUCLEIC-ACID STRUCTURES | - |
dc.subject.keywordPlus | PROTEIN COMPLEX | - |
dc.subject.keywordPlus | FISSION YEAST | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | RAD51 RECOMBINASE | - |
dc.subject.keywordPlus | DNA RECOMBINATION | - |
dc.subject.keywordPlus | HOP2 PROTEIN | - |
dc.subject.keywordPlus | MEIOSIS | - |
dc.subject.keywordPlus | DMC1 | - |
dc.subject.keywordPlus | DOUBLE-STRAND BREAKS | - |
dc.subject.keywordPlus | NUCLEIC-ACID STRUCTURES | - |
dc.subject.keywordPlus | PROTEIN COMPLEX | - |
dc.subject.keywordPlus | FISSION YEAST | - |
dc.subject.keywordPlus | SACCHAROMYCES-CEREVISIAE | - |
dc.subject.keywordPlus | RAD51 RECOMBINASE | - |
dc.subject.keywordPlus | DNA RECOMBINATION | - |
dc.subject.keywordPlus | HOP2 PROTEIN | - |
dc.subject.keywordPlus | MEIOSIS | - |
dc.subject.keywordPlus | DMC1 | - |
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