Heterogeneous nuclear ribonucleoproteins C1 and C2 associate with the RNA component of human telomerase

Cited 64 time in webofscience Cited 68 time in scopus
  • Hit : 235
  • Download : 0
Here we demonstrate that heterogeneous nuclear ribonucleoproteins (hnRNPs) C1 and C2 can associate directly with the integral RNA component of mammalian telomerase. The binding site for hnRNPs C1 and C2 maps to a 6-base uridylate tract located directly 5' to the template region in the human telomerase RNA #TR# and a 4-base uridylate tract directly 3' to the template in the mouse TR. Telomerase activity is precipitated with antibodies specific to hnRNPs C1 and C2 from cells expressing wild-type human TR but not a variant of the human TR lacking the hnRNPs C1 and C2 binding site, indicating that hnRNPs C1 and C2 require the 6-base uridylate tract within the human TR to associate with the telomerase holoenzyme. In addition, we demonstrate that binding of hnRNPs C1 and C2 to telomerase correlates with the ability of telomerase to access the telomere. Although correlative, these data do suggest that the binding of hnRNPs C1 and C2 to telomerase may be important for the ability of telomerase to function on telomeres. The C proteins of the hnRNP particle are also capable of colocalizing with telomere binding proteins, suggesting that the C proteins may associate with telomeres in vivo. Therefore, human telomerase is capable of associating Kith core members of the hnRNP family of RNA binding proteins through a direct and sequence specific interaction with the human TR. This is also the first account describing the precise mapping of a sequence in the human TR that is required to associate with an auxiliary component of the human telomerase holoenzyme.
Publisher
AMER SOC MICROBIOLOGY
Issue Date
2000-12
Language
English
Article Type
Article
Keywords

HELIX-UNWINDING PROTEINS; IMMORTAL HUMAN-CELLS; IN-VITRO; CALF THYMUS; BINDING-SPECIFICITY; CATALYTIC SUBUNIT; HNRNP PROTEINS; LIFE-SPAN; DNA; END

Citation

MOLECULAR AND CELLULAR BIOLOGY, v.20, no.23, pp.9084 - 9091

ISSN
0270-7306
DOI
10.1128/MCB.20.23.9084-9091.2000
URI
http://hdl.handle.net/10203/198296
Appears in Collection
MSE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 64 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0