DC Field | Value | Language |
---|---|---|
dc.contributor.author | Lee, Jeong Soon | ko |
dc.contributor.author | Suh, Jae Myoung | ko |
dc.contributor.author | Park, Hong Gyu | ko |
dc.contributor.author | Bak, Eun Jung | ko |
dc.contributor.author | Yoo, Yun-Jung | ko |
dc.contributor.author | Cha, Jeong-Heon | ko |
dc.date.accessioned | 2015-04-29T01:20:17Z | - |
dc.date.available | 2015-04-29T01:20:17Z | - |
dc.date.created | 2015-04-24 | - |
dc.date.created | 2015-04-24 | - |
dc.date.issued | 2008-06 | - |
dc.identifier.citation | DIFFERENTIATION, v.76, no.5, pp.478 - 487 | - |
dc.identifier.issn | 0301-4681 | - |
dc.identifier.uri | http://hdl.handle.net/10203/198276 | - |
dc.description.abstract | Adipocytokines, bioactive molecules secreted from adipose tissues, play important roles in physiology, development, and disease. Recently, heparin-binding epidermal growth factor-like growth factor (HB-EGF) was identified as an adipocytokine whose expression correlates with obesity. However, the biological role of fat-secreted HB-EGF is still unclear. In this study, we investigated the effects of HB-EGF on the adipocyte differentiation of C3H10T1/2 pluripotent mesenchymal cells. Upon adipogenic conversion of C3H10T1/2 cells, HB-EGF displayed dynamic changes in expression where an initial decrease was followed by increased levels of expression at later stages. HB-EGF treatment during adipogenic induction inhibited lipid accumulation and decreased the expression of adipocyte molecular markers (fatty acid-binding protein, peroxisome proliferator-activated receptor gamma, and CAAT enhancer-binding protein alpha) and lipogenic genes (glucose transporter, fatty acid synthetase, and lipoprotein lipase). Therefore, HB-EGF has an inhibitory effect on adipocyte differentiation. Administration of HB-EGF at various intervals during adipocyte differentiation revealed that HB-EGF acts during the early stages of adipocyte differentiation, but not at the later stages of differentiation. Furthermore, HB-EGF was able to block the commitment of pluripotent mesenchymal cells to the adipocyte lineage triggered by bone morphogenic protein 4 treatment. These data suggest that HB-EGF acts as a negative regulator of adipogenesis by inhibiting the commitment and early differentiation of the adipose lineage. The inhibitory role of HB-EGF on adipocyte differentiation of pluripotent mesenchymal cells sheds light on potential mechanisms that control adipose tissue homeostasis. | - |
dc.language | English | - |
dc.publisher | BLACKWELL PUBLISHING | - |
dc.subject | MESENCHYMAL STEM-CELLS | - |
dc.subject | MITOTIC CLONAL EXPANSION | - |
dc.subject | INSULIN-RESISTANCE | - |
dc.subject | ADIPOSE CONVERSION | - |
dc.subject | HB-EGF | - |
dc.subject | DIABETES-MELLITUS | - |
dc.subject | IN-VITRO | - |
dc.subject | OBESITY | - |
dc.subject | EXPRESSION | - |
dc.subject | MOUSE | - |
dc.title | Heparin-binding epidermal growth factor-like growth factor inhibits adipocyte differentiation at commitment and early induction stages | - |
dc.type | Article | - |
dc.identifier.wosid | 000256117500004 | - |
dc.type.rims | ART | - |
dc.citation.volume | 76 | - |
dc.citation.issue | 5 | - |
dc.citation.beginningpage | 478 | - |
dc.citation.endingpage | 487 | - |
dc.citation.publicationname | DIFFERENTIATION | - |
dc.identifier.doi | 10.1111/j.1432-0436.2007.00250.x | - |
dc.contributor.localauthor | Suh, Jae Myoung | - |
dc.contributor.nonIdAuthor | Lee, Jeong Soon | - |
dc.contributor.nonIdAuthor | Park, Hong Gyu | - |
dc.contributor.nonIdAuthor | Bak, Eun Jung | - |
dc.contributor.nonIdAuthor | Yoo, Yun-Jung | - |
dc.contributor.nonIdAuthor | Cha, Jeong-Heon | - |
dc.type.journalArticle | Article | - |
dc.subject.keywordAuthor | HB-EGF | - |
dc.subject.keywordAuthor | BMP4 | - |
dc.subject.keywordAuthor | C3H10T1/2 | - |
dc.subject.keywordAuthor | mesenchymal stem cell | - |
dc.subject.keywordAuthor | adipocyte differentiation | - |
dc.subject.keywordAuthor | EGF receptor | - |
dc.subject.keywordAuthor | mitotic clonal expansion | - |
dc.subject.keywordAuthor | C/EBP alpha | - |
dc.subject.keywordAuthor | PPAR gamma | - |
dc.subject.keywordAuthor | aP2 | - |
dc.subject.keywordPlus | MESENCHYMAL STEM-CELLS | - |
dc.subject.keywordPlus | MITOTIC CLONAL EXPANSION | - |
dc.subject.keywordPlus | INSULIN-RESISTANCE | - |
dc.subject.keywordPlus | ADIPOSE CONVERSION | - |
dc.subject.keywordPlus | HB-EGF | - |
dc.subject.keywordPlus | DIABETES-MELLITUS | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | OBESITY | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | MOUSE | - |
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