Identification of novel genes responsible for salt tolerance by transposon mutagenesis in Saccharomyces cerevisiae

Cited 4 time in webofscience Cited 0 time in scopus
  • Hit : 192
  • Download : 0
Saccharomyces cerevisiae strains tolerant to salt stress are important for the production of single-cell protein using kimchi waste brine. In this study, two strains (TN-1 and TN-2) tolerant of up to 10 % (w/v) NaCl were isolated by screening a transposon-mediated mutant library. The determination of transposon insertion sites and Northern blot analysis identified two genes, MDJ1 and VPS74, and revealed disruptions of the open reading frame of both genes, indicating that salt tolerance can be conferred. Such tolerant phenotypes reverted to sensitive phenotypes on the autologous or overexpression of each gene. The two transposon mutants grew faster than the control strain when cultured at 30 A degrees C in rich medium containing 5, 7.5 or 10 % NaCl. The genes identified in this study may provide a basis for application in developing industrial yeast strains.
Publisher
SPRINGER HEIDELBERG
Issue Date
2015-04
Language
English
Article Type
Article
Keywords

ETHANOL TOLERANCE; GLYCEROL-3-PHOSPHATE DEHYDROGENASE; MITOCHONDRIAL-DNA; WASTE-WATER; YEAST; STRESS; EXPRESSION; PROTEIN; DISRUPTION; GROWTH

Citation

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, v.42, no.4, pp.567 - 575

ISSN
1367-5435
DOI
10.1007/s10295-015-1584-y
URI
http://hdl.handle.net/10203/198217
Appears in Collection
CBE-Journal Papers(저널논문)
Files in This Item
There are no files associated with this item.
This item is cited by other documents in WoS
⊙ Detail Information in WoSⓡ Click to see webofscience_button
⊙ Cited 4 items in WoS Click to see citing articles in records_button

qr_code

  • mendeley

    citeulike


rss_1.0 rss_2.0 atom_1.0