Microfluidic device for manipulation and lysis of bacterial cells using nanowire array = 나노선 어레이를 이용한 박테리아 세포의 조작과 용해를 위한 미세유체소자

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In this master’s thesis, we present a microfluidic device integrated with ZnO nanowire array that aims for rapid detection and analysis of bacterial cells towards widespread use in real fields applications. The proposed detection and analysis of bacterial cells involves three steps: (1) cell manipulation, (2) cell lysis, and (3) detection and analysis of DNA. First, cell manipulation step is performed by hydrodynamic trapping of bacterial cells in the microfluidic device using fishnet-like cage structure array. The fishnet-like cage structures are fabricated by integration of ZnO nanowire array into microslit structures. Since ZnO nanowires synthesized by hydrothermal method are dense and robust enough to endure the moving bacterial cells, they can be trapped at the fishnet-like cages within the microfluidic device without any complicated experimental setup and delicate system control. Second, cell lysis step is conducted by synergic cell wall damage of bacterial cells based on both hydrodynamic (shear-force based) lysis and photocatalytic lysis. For hydrodynamic lysis, moving bacterial cells in the microfluidic device experience collision and friction with ZnO nanowire array. ZnO nanowires are used as the mechanical scratching sources based on their geometry characterized by sharp tips and high aspect ratio. For photocatalytic lysis, bacterial cell wall is disrupted by oxidative damage in the presence of ZnO nanowires and UV light. Through these lysis mechanisms, we can collect DNA for further assay. Lastly, detection and analysis of DNA is achieved by PCR based method which amplifies DNA templates including target sequence after extraction of them from target bacterial cells. It can be demonstrated that the proposed sample preparation strategy can be used for PCR based detection method without serious degradation of DNA templates.
Advisors
Park, In-Kyuresearcher박인규
Description
한국과학기술원 : 기계공학전공,
Publisher
한국과학기술원
Issue Date
2014
Identifier
568777/325007  / 020123034
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 기계공학전공, 2014.2, [ ⅶ, 62 p. ]

Keywords

Microfluidic Device; PCR 기반 검출법; 세포 용해; 박테리아; 산화아연 나노선; 미세유체소자; ZnO Nanowires; Bacteria; Lysis; PCR based Detection

URI
http://hdl.handle.net/10203/197526
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=568777&flag=dissertation
Appears in Collection
ME-Theses_Master(석사논문)
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