Electrochemical study of thrombin/dna detection using scanning electrochemical microscopy(SECM) and carbon nanotube/aptamer/PNA = 전기화학적 주사현미경과 탄소나노튜브/앱타머/PNA를 이용한 트롬빈/DNA의 검출 및 전기화학적 연구

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The electrochemical methods are widely used in many sensing research areas. In this dissertation, the electrochemical methods were used to study a sensitive thrombin/DNA detection method using scanning elec-trochemical microscopy (SECM), single wall carbon nanotube (SWCNT) casted glassy carbon electrode, thrombin binding aptamer (TBA) and peptide nucleic acid (PNA). An electrochemical aptasensor with a thrombin binding aptamer (TBA) was developed using a single wall carbon nanotube (SWCNT) casted GCE. To detect the target molecules, aptamers are currently focused on and the use of aptamers for biosensing is particularly interesting, as aptamers could substitute antibodies in bioanalytical sensing. The SWCNT casted GCE provides a suitable conducting matrix for TBA immobiliza-tion and electrochemical detection. The TBA was immobilized on SWCNTs through π-stacking without any special modification, resulting in helical wrapping to the surface. In the presence of thrombin, the TBA binds with thrombin and the TBA concentration on the SWCNT surface decreases. Therefore, the binding event between the TBA and thrombin results in decreased concentration of the TBA on the SWCNT surface. The remaining amount of TBA can be analyzed by an electrochemical method without any label, which is com-monly used for signal detection and amplification in electrochemical biosensors, because the guanine bases of the nucleic acid are measurable by electrochemical methods. The electrochemical oxidation of guanine nucleotides was enhanced by electrocatalytic mediation using Ru(bpy)32+ for higher sensitivity and reduction of the overpotential for electrochemical detection. This dissertation describes the novel electrochemical system for protein in sandwich manner by using the aptamers and the scanning electrochemical microscope (SECM). For protein detection, sandwich system is ideal since labeling of the target protein is not necessary. To develop the electrochemical protein sensor sys-te...
Advisors
Kwak, Ju-Hyounresearcher곽주현
Description
한국과학기술원 : 화학과,
Publisher
한국과학기술원
Issue Date
2014
Identifier
568643/325007  / 020085068
Language
eng
Description

학위논문(박사) - 한국과학기술원 : 화학과, 2014.2, [ ix, 80 p. ]

Keywords

Thrombin; 전기화학적 바이오센서; 전기화학적 주사 현미경; 트롬빈 결합 앱타머; 트롬빈; Electrochemical biosensor; Thrombin binding aptamer(TBA); Peptide nucleic acid(PNA); Scanning electrochemical microscopy(SECM)

URI
http://hdl.handle.net/10203/196452
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=568643&flag=dissertation
Appears in Collection
CH-Theses_Ph.D.(박사논문)
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