High-yield purification of rare blood cells by immunospecific size enhancement in an inertial microfluidic device = 면역특이적으로 크기가 증대된 혈중 희귀세포의 고순도 회수를 위한 관성 미세유체소자 내에서의 분리법

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Circulating rare cells in blood provide valuable information and their separation can open opportuni-ties for biological studies. Separation of circulating tumor cells (CTCs) in specific can be used as a non-invasive liquid biopsy and biomarker for cancer diagnosis and screening purposes. CTCs are generally larger than white blood cells (WBCs) and many size-based separation methods utilizing inertial microfluidics for high throughput separation have been developed in recent years. However, due to the overlap in size distribu-tions between WBCs and CTCs, it has been difficult to separate with both high recovery rate and purity. In this study, to overcome the limitation of size-based inertial separation of CTCs from WBCs, immunospecific size enhancement was applied to eliminate that size overlap. To demonstrate improved separation, mi-crobeads are attached to CTCs by antigen-antibody binding to enhance their sizes, and then separated from WBCs in an inertial microfluidic separating device that consists of a series of contraction-expansion arrays (CEA). In addition, the dimensions of CEA device were systematically varied for optimal separation performance and computational fluid dynamics simulations were performed to characterize the secondary flows in the device. Breast cancer and monocyte cancer cell lines were chosen to model CTCs and WBCs inertial migration in the optimized device, respectively, and then breast cancer cells were labeled with beads to compare the separation performance. The device separates unlabeled CTCs from WBCs with 74% recovery rate and 76% WBC rejection ratio at 0.88 ml/h. However, increased recovery rate of 97.6% and 95% WBC rejection ratio at 0.88 ml/h was demonstrated by labeling the CTCs with 5 μm beads. With increased recovery rate and purity provided by increased WBC rejection ratio, inertial separation of bead-labeled CTCs not only has the potential to improve the clinical diagnostic, prognostic and screening results, but also to ...
Park, Je-Kyunresearcher박제균
한국과학기술원 : 바이오및뇌공학과,
Issue Date
568890/325007  / 020123376

학위논문(석사) - 한국과학기술원 : 바이오및뇌공학과, 2014.2, [ xi, 73 p. ]


Inertial microfluidics; 혈중 암세포; 혈중 희귀세포; 세포분리; 이차유동장; 관성 유체역학; Secondary flow; Cell separation; Circulating rare cells; Circulating tumor cells

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