A study on microalgae hydrolysis and ethanol production by a recombinant Streptomyces lividans TK24 = 미세조류 당화와 이의 당화물로부터 Streptomyces lividans TK24를 이용한 에탄올 생산에 관한 연구

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The production of bioethanol from microalgal biomass was studied. The whole process was comprised of two steps. In the first step, harvested Nannochloropsis oceanica cell mass was hydrolyzed by using an acid. In the second step, ethanol was produced by a recombinant Streptomyces lividans TK24 from the hydrolysate. In the preliminary study, the acid hydrolysate was found to contain 7 different sugars of glucose, galactose, xylose, rhamnose, mannose, ribose, and fucose with negligible amount of toxic compounds that inhibit microbial activity. Among three acids of HNO3, HCl, and H2SO4 tested for the hydrolysis of microalgal biomass, HNO3 was found to be most effective and thus selected for the subsequent experimental work. To optimize the hydrolysis conditions, effects of acid concentration and reaction temperature on hydrolysis were investigated. The highest sugar yield of 78 % was obtained at 90 min when the acid concentration and the reaction temperature were 2N and 90oC, respectively. A S. lividans TK24 strain transformed with the pyruvate decarboxylase (pdc) and alcohol dehydrogenase Ⅱ (adhⅡ) genes was used for ethanol fermentation. It could metabolize various carbon sources including above-mentioned sugars contained in the microalgal hydrolysate except for fucose. When a simulated acid hydrolysate containing in g/L glucose 10, galactose 8, xylose 3, rhamnose 1, mannose 5 and ribose 3 (30 g/L in total), 5.1 g/L of ethanol was produced.
Advisors
Chang, Yong-Keunresearcher장용근
Description
한국과학기술원 : 생명화학공학과,
Publisher
한국과학기술원
Issue Date
2013
Identifier
566476/325007  / 020123693
Language
eng
Description

학위논문(석사) - 한국과학기술원 : 생명화학공학과, 2013.8, [ vii, 53 p. ]

Keywords

N. oceanica; 에탄올; 유전자 재조합 방선균; 산 분해; N. oceanica; Ethanol; Acid hydrolysis; Recombinant S. lividans

URI
http://hdl.handle.net/10203/196249
Link
http://library.kaist.ac.kr/search/detail/view.do?bibCtrlNo=566476&flag=dissertation
Appears in Collection
CBE-Theses_Master(석사논문)
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